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Standards, Tools, and Databases for the Analysis of Yeast Omics Data
Time of Update: 2021-02-22
Biological data and software tools for the design, analysis, and simulation of models are two basic ingredients for the new field of systems biology.
We also provide a review of the most popular software tools currently used in computational systems biology studies.
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Transient and Inducible Expression of Vaccinia/T7 Recombinant Viruses
Time of Update: 2021-02-22
Transient expression can then be achieved either by transfecting this plasmid into cells infected with the recombinant vaccinia virus expressing T7 RNA polymerase, vTF7-3 or by crossing this plasmid into the vaccinia virus genome and coinfecting cells with both viruses.
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Isolation of Yeast Mitochondria
Time of Update: 2021-02-22
Often preparations of isolated organelles contain other, unwanted, cellular components.
For these types of studies, it is crucial to have highly pure fractions of the desired organelle.
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Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers
Time of Update: 2021-02-22
The standard approach for the isolation of vaccinia virus recombinants involves homologous recombination between a transfected plasmid and the replicating viral DNA .
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Transformation of Lactococcus by Electroporation
Time of Update: 2021-02-22
lactis employed protoplast transformation in the presence of polyethylene glycol ( 1 ).
Later work indicated that the cell wall is a physical barrier to the entering DNA not only during protoplast transformation, but also during electroporation.
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Identification of Yeasts Present in Sour Fermented Foods and Fodders
Time of Update: 2021-02-22
The main products of this bacterial process are lactic and acetic acids, which are toxic to many microorganisms, most yeasts included.
This application of the lactic acid fermentation originated from central Asia in times immemorial and has spread from there to Europe and the Orient.
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Design and Construction of Poliovirus Epitope Expression Vectors
Time of Update: 2021-02-22
We have developed the very safe and efficacious live-attenuated Sabin 1 poliovirus vaccine strain as a vehicle for the presentation of defined epitopes from foreign pathogens ( 1 – 3 ). Precise modif
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Quality Criteria in Pesticide Analysis
Time of Update: 2021-02-22
There is an increasing concern in pesticide residue analysis laboratories to ensure the quality of their analytical results.
Internal quality control (I QC ) measures are an essential element to ensure reliable results because they allow both the continuous monitoring of the process and measurements and the elimination of causes of unsatisfactory performance.
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Detection of Mutations in Mycobacteria by PCR-SSCP (Single-Strand Conformation Polymorphism)
Time of Update: 2021-02-22
f the available techniques, single-strand conformation polymorphism analysis (SSCP) appears as one of the most versatile techniques, amenable to automation, and optimal for screening for known or unknown mutations.
DNA duplexes(25) Single-strand conformation polymorphism (SSCP)(1)
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Neutralization of the In Vivo Activity of E. Coli-Derived Lipopolysaccharide by Cationic Peptides
Time of Update: 2021-02-22
Lipopolysaccharide-binding and neutralization of its biologic activity can be detected and quantified by monitoring specific in vivo responses induced by intravascular introduction of lipopolysaccharide into conscious, instrumented animals.
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Isolation and Characterization of Extrachromosomal Double-Stranded RNA Elements in Xanthophyllomyces dendrorhous
Time of Update: 2021-02-22
Double-stranded RNA (dsRNA) molecules are widely found in yeasts and filamentous fungi.
In this chapter, we describe a simple method to isolate dsRNA elements from yeasts using low amounts of starting material, and their application to differentXanthophyllomyces dendrorhous strains.
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Insertion Element IS6110-Based Restriction Fragment Length Polymorphism Genotyping of Mycobacterium tuberculosis
Time of Update: 2021-02-22
DNA fingerprinting techniques are based on genome variation and form the basis of molecular epidemiology studies of tuberculosis.
A number of markers are in use for the molecular differentiation ofMycobacterium tuberculosis isolates by DNA fingerprinting.
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Cytokines, Stress, and Depressive Illness
Time of Update: 2021-02-22
Indeed, it appears that in many respects the effects of stressors are reminiscent of the presumed neurochemical disturbances thought to be associated with depression, including elevated hypothalamic-pituitary-adrenal (HPA) functioning, as well as altered monoamine activity within hypothalamic and limbic areas of the brain.
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Expression of Tissue Inhibitor of Metalloproteinases by Recombinant Baculovirus-Infected Insect Cells Cultured in an Airlift Fermentor
Time of Update: 2021-02-22
In this system, the gene to be expressed is cloned into a plasmid transfer vector, downstream of a strong baculovirus gene promoter, and cotransfected with wildtype virusDNA into insect cells.
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Virus-like Particle and Viral Vector Production Using the Baculovirus Expression Vector System/Insect Cell System: Adeno-Associated Virus-Based Products
Time of Update: 2021-02-22
The ability to make a large variety of virus-like particles (VLPs) has been successfully achieved in the baculovirus expression vector system (BEVS)/insect cell system.
This chapter focuses on the methodology, based on the work done in our lab, for the production of AAV-like particles and vectors in a BEVS/insect cell system.
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In Vitro Methods to Study RNA Interference During an Adenovirus Infection
Time of Update: 2021-02-22
RNA interference (RNAi) has attracted a lot of interest during recent years as a method to knock-down gene expression and as a possible antiviral system. Here we present a collection of in vitro meth
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Detection and Differentiation of Ruminant Mycoplasmas
Time of Update: 2021-02-22
While a considerable part of this group is conceived to be of minor epidemiological relevance, it contains some important pathogenic agents that have specific host ranges.
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Tn611 Transposon Mutagenesis in Mycobacterium smegmatis Using a Temperature-Sensitive Delivery System
Time of Update: 2021-02-22
Mobile generic elements are discreteDNA fragments encoding their own transposltlon functions, which enable them to move from one site in the DNA to another, independently of host-recombination functions (such as RecA).
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Molecular Detection of Drug-Resistant Mycobacterium tuberculosis with a Scanning-Frame Oligonucleotide Microarray
Time of Update: 2021-02-22
In this chapter, we describe the utility of a microarray-based approach exploiting short overlapping oligonucleotides (sliding-frame array) to rapidly detect drug resistance–associated mutations (substitutions, deletions, and insertions) in thepncA gene responsible for resistance of M.
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Analysis of Cytotoxic T-Cell Responses to Human Cytomegalovirus Infection
Time of Update: 2021-02-22
Inferential evidence from studies of immunosuppressed humans indicates the control of disease due to human cytomegalovirus (HCMV) in the persistently infected host (virus carrier) depends on the cell
