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Virus air spot experiment question and answer
Time of Update: 2021-01-20
However, methyl cellulose to be configured long in advance, about 2 weeks, after high pressure into the refrigerator, before use sterile operation to add culture fluid, mixed into the refrigerator, after a day to take out, shake hard, so that cellulose to achieve a homogeneous state, sit until the blister completely disappeared after use.
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Blood clotting inhibition test
Time of Update: 2021-01-20
can be assumed that 90××, 100×, 120×, 130× are 4HAU, 2 × , 3×, 4×, 5×, 6× dilution, each 4 holes, and red blood cells and virus control, according to this to do a blood clotting experiment, so as to determine 4 units of antigen.
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Introduction and application of lyovirus packaging system
Time of Update: 2021-01-20
In order to produce high-titular virus particles, it is necessary to use the expression vector and packaging protons at the same time transposed cells, in the cell for viral packaging, packaged fake virus particles secreted to the culture base outside the cell, centrifugation can be directly used for the host cell infection, the destination gene into the host cell, after reverse transcription, integration into the genome , so as to achieve a high level of expression molecules.
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Bacterial mutants are constructed through homogeneity recombination of suicidal protons
Time of Update: 2021-01-20
The mutant gene is recombined with the wild type on the host chromosome, resulting in a mutant strain with a mutation, while the prosurge vector itself disappears from the bacteria along with the original wild type gene on the chromosome due to its suicidal properties.
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Microbiological identification methods
Time of Update: 2021-01-20
I. Microbiology Identification Methods - Traditional Methods In the traditional classification identification, the main basis of microbial classification identification is morphological characteristics, physiological and bio-reaction characteristics, ecological characteristics, as well as serynological response, sensitivity to phages and so on.
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Simple determination of bacterial concentration: Macy's than turbidity
Time of Update: 2021-01-20
Macbeth turbidity tube is a standard turbidity tube invented by McFarland for "> microorganisms different turbidity than turbidity. The specific preparation method is based on the ratio of sulfuric
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Preparation of a certain concentration of bacterial suspension
Time of Update: 2021-01-20
(ii) Basic Principles The microscope direct counting method is a simple, fast and intuitive way to place the suspension of a small sample to be measured on a special slide with a defined area and volume (also known as a fungicide) and count directly under the microscope.
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E. coli detection method
Time of Update: 2021-01-20
4. Inspection procedures: samples dilution lactose bile salt fermentation tube, 36±1 degrees C, 24±2hr No gas production coliococcal group negative Ihong Meilan agar plate, 36±1 degrees C, 24±2hr report -stained lactose fermentation tube, 36±1 degree c, 24±2hr , G-G-, spore-free gas-producing -- E.
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Principles and precautions of sulfur ethanolate fluid media
Time of Update: 2021-01-20
media after sterilization to use the process should avoid shaking, so as to avoid the culture gene vibration into oxygen, affecting the use of media, vaccination operation should be rapid, agile, otherwise exposed to the air too long, the media is oxidized.
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Beef paste protein 胨 the characteristics and production steps of the culture base
Time of Update: 2021-01-20
beef paste protein the formulation of the culture base is as follows: < "> protein 10gNaCl 5g agar 15-20g water 1000ml pH 7． 4—7． 6 , equipment< "text-align:left;"> beef paste, protein, NaCl, agar; 1mol/L NaOH, 1mol/L HCl; test tube, triangular burner, beaver, tube, stick, Medium displodator, torque balance, horn spoon, high-pressure steam sterilization pot, pH "test paper (pH 5.
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The advantages and disadvantages of each species preservation method
Time of Update: 2021-01-20
1． Slope low temperature preservation method (1) inoculated the bacteria in a suitable solid slope culture base, to be fully grown, the cotton plug part with oil paper wrapped, moved to 2-8 degrees C refrigerator storage.
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Isolated culture of plant pathogenic fungi
Time of Update: 2021-01-20
the separation of plant pathogenic fungi is generally the use of tissue separation method, that is, the cutting of small pieces of diseased tissue, after surface disinfection and sterilization of water wash, moved to artificial flisting base culture.
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The preparation of LB cultures
Time of Update: 2021-01-20
LB culture base preparation: ingredients tryptone 10g yeast extract 5g sodium chloride (NaCl) 10g Solid media plus agar powder 15-20g plus double steamed water to 1000mL, pH to 7.2,121C sterilization 30min preparation method (1) weighing the required amount of tryplin , yeast extract and NaCl, respectively, placed in berries.
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Phage culture
Time of Update: 2021-01-20
the phage body progenitor 100 sl is evenly mixed with the host bacteria suspension liquid for 300 sl and remained in place for 15 minutes to infect it.
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The PCR method detects myosomes
Time of Update: 2021-01-20
(2) template production: in sterile conditions, take cell culture on clear 1 00ul in a sterile 0.5 ml plastic centrifuge tube, covered with a lid, 95 degrees C water bath heating 5min.
(6) takes a centrifugal tube containing the above reaction system and adds 5ul deionized water as a negative control tube.
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Massive amplification of adenovirus in 293 cells
Time of Update: 2021-01-20
-200C/370C freezes and melts 3 times, and the desktop centrifuge centrifuges remove cell fragments at the maximum rate, collect them, and then titration the virus.
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Phage demonstration technology
Time of Update: 2021-01-20
The main feature of this technique is to unify the genotypes and ideotypes of a particular molecule within the same virus particle, i.e., to display a specific protein on the surface of the phage, and to contain the structural genes of the protein in the core DNA of the phage.
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How-to guide for lyovirus use
Time of Update: 2021-01-20
The third day, change the culture fluid: generally after 24 years of childhood will contain lysovirus culture liquid replaced with normal culture fluid, after infection to observe the state of the cell, if the lyovirus has a significant toxic effect on the cell and affect the cell growth state, can be as short as the virus 4 childhood replacement of fresh culture solution continued culture (recommended in 8-12 hours replacement is appropriate).
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Testing of fecal colium
Time of Update: 2021-01-20
2． 44 degrees C lactose fermentation test the sample inoculated with sterile operation in the lactose bile salt fermentation tube (1 mL or more 1 mL and 1 mL or less, with a single lactose bile salt fermentation tube), placed (44 soil 0.5) in the water bath, culture (24 soil 2) h.
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Vaccination, separation purification and culture techniques
Time of Update: 2021-01-20
, vaccination microorganisms to the artificial culture suitable for its growth and reproduction or the process of living organisms is called inoculation.
This method is to inoculate a small number of microorganisms on the surface of the plate, into three points of egaldiscular triangles, so that it independently forms bacteria backward, to observe and study their form.
