Vaccination, separation purification and culture techniques

, vaccination

microorganisms
to the artificial
culture suitable for its growth and reproduction
or the process of living organisms is called inoculation.
1, vaccination tools and methods
in laboratory or factory practice, the most used vaccination tools are vaccination rings, vaccination needles. Because of the different vaccination requirements or methods, the needle tip of the vaccination needle is often made into different shapes, there are knife-shaped, tweezers and so on. Sometimes dropper,
straw
can also be used as an inoculation tool for liquid inoculation. In solid
,
need to be used when applying the liquid evenly to the surface of the solid culture. (Figure 3-3)Figure 3-3 Inoculation and separation tools
Inoculation needle 2. Vaccination ring 3. Inoculation hook 4.5. Glass stick 6. Inoculation ring 7. Inoculation ring 8. Small anatomy knife
There are several commonly used vaccination methods:
1) line vaccination This is the most commonly used vaccination method. That is, on the solid culture base surface for a straight-forward movement back and forth, you can achieve the role of inoculation. Commonly used vaccination tools are vaccination rings, vaccination needles, etc. This method is commonly used in bevel inoculation and flat-panel dashing.
2) Three-point vaccination is commonly used in the study of mold forms. This method is to inoculate a small number of microorganisms on the surface of the plate, into three points of egaldiscular triangles, so that it independently forms bacteria backward, to observe and study their form. In addition to three points, there are one or more inoculations.
3) Puncture inoculation is often used in the motivation to preserve anaerobic strains or to study microorganisms. When you do puncture vaccination, the vaccination tool used is the vaccination needle. The culture base used is generally a semi-solid culture base. It is done: with inoculation needles to extract a small number of strains, along the semi-solid culture center to the bottom of the tube for a straight puncture, if a bacterium has whiplash and can move, can grow around the puncture line.
4) The method of pouring and inoculation is to put the microorganisms to be picked up in a petri dish, and then pour into a solid medium cooled to about 45 degrees C, quickly and gently shake, so that the liquid can achieve the purpose of dilution. After the plate solidified, culture at a suitable temperature, you can grow a single microbial microbial bacterios.
5) coating inoculation and watering inoculation slightly different, is to first pour the plate, let it solidify, and then pour the bacteria liquid into the plate above, quickly with the coating stick on the surface for back and forth coating, so that the liquid evenly distributed, you can grow a single microbial bacteria.
6) Liquid inoculation It can be called liquid inoculation by washing the bacteria from a solid medium, pouring them into a liquid medium, or by connecting the bacteria to the liquid medium with a pipet, or by moving the bacteria from a liquid culture to a solid medium.
7) Vaccination Is the method of injecting the waiting microorganisms into living organisms, such as humans or other animals, the common vaccination, is to use injection, access to the human body, to prevent certain diseases.
8) Live inoculation is a method specifically designed to culture viruses or other pathogenic microorganisms, as viruses must be inoculated in living organisms to grow and reproduce. The living body used can be an entire animal, or it can be an
tissue
, such as a monkey kidney, or it can be a developing chicken embryo. Vaccination can be given by injection or mixed feeding.
2, sterile operation
culture base by
autocultension
, with sterilized tools (such as inoculation needles and straws, etc.) in sterile conditions inoculated bactericial materials (such as samples, moss or bacterial suspension, etc.) on the culture base, this process is called sterile inoculation operation. Various vaccinations in laboratory tests must be sterile.
test bench
, no matter what material, all require smooth, horizontal. Smoothness is easy to scrub with disinfectant, and the level is
the
agar" culture base to facilitate the thickness of the plate inside the petri dish. At the experimental table, the air flow should be slow, germs should be minimized, and the fewer clutter around them, the better. To do this, the room must be cleaned, the doors and windows of the laboratory closed, and air disinfected with disinfectant to minimize the number of germs.