Principles and precautions of sulfur ethanolate fluid media

I, role:
sulphate fluid
culture
base is mainly used for the cultivation of aerobic, anaerobic and micro-oxygen
microbials
, is the first choice for the cultivation of anaerobic bacteria
train
. The medium has been used as a sterile examination medium in pharmacopeia and has an important role to play.
II, principle:
the main components of the fluid culture: casein , yeast leaching powder, glucose, sodium chloride, L-cysteine (cysteine), sodium thioethanolate (sulfur ethanolate 0.6 mL),
agar
, bladed green and so on.
among them, casein , yeast leaching powder provides nitrogen sources and a variety of amino acids and
growth factors
necessary for synthesis of
proteins
glucose provides carbon sources and energy; Toxicity, conducive to the growth of anaerobic bacteria, in addition, sulfur ethanolate has a passivated arsenic and mercury-containing drugs and other metals
septics
harmful effects;
III, the characteristics of sulfur ethanolate fluid media:
from the sulfur ethanolate fluid media composition analysis can be concluded that the characteristics of the media are:
contains more comprehensive nutrition, can meet the nutritional needs of the vast majority of bacteria;
The different depth upper, middle and lower parts of the medium can be simulated to provide aerobic, weak oxygen to anaerobic different oxygen content of the gradient environment, so that anaerobic bacteria, anaerobic bacteria, aerobic bacteria can grow in this medium


4. Precautions when using:
when making sulfur ethanolate fluid media, you need to pay attention to the following issues:
sulfur ethanolate fluid media has a small amount of agar, after sterilization, the media should be presented without turbidity, if placed for a period of time, there is slight turbidity, especially after putting in the refrigerator, turbidity phenomenon is more obvious. The degree of turbidity may be related to the quality of agar used in the culture on the one hand, or to the thoroughness of sterilization on the other, and should be analyzed in detail. If the former may consider replacing the culture base manufacturer or batch number, or the latter should be checked for careful sterilization operation and sterilization equipment.
media after sterilization to use the process should avoid shaking, so as to avoid the culture gene vibration into oxygen, affecting the use of media, vaccination operation should be rapid, agile, otherwise exposed to the air too long, the media is oxidized.
Before inoculation, if the color of the oxidizing layer (pink) exceeds 1/5 of the depth of the medium, boil
heat
to pink (no more than 20 min) and cool quickly. The medium is heated only once to avoid a decrease in the quality of the medium.
-edged tianqing should be fresh preparation, otherwise affect the judgment.
Li Chao
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