Insertion Element IS6110-Based Restriction Fragment Length Polymorphism Genotyping of Mycobacterium tuberculosis
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Last Update: 2021-02-22
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Source: Internet
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Author: User
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DNA
fingerprinting techniques are based on genome variation and form the basis of molecular epidemiology studies of tuberculosis. A number of markers are in use for the molecular differentiation of
Mycobacterium tuberculosis
isolates by DNA fingerprinting. One of these markers is the IS
6110
insertion element, which may be present in up to 25 copies per
M. tuberculosis
genome. Variation in both the number and location of the IS
6110
elements makes it a very useful marker of strain genotype. IS
6110
-based DNA fingerprinting is globally considered as the reference genotyping technique for
M. tuberculosis
isolates. This method is based on visualization of restriction fragment length polymorphisms using a labeled probe derived from IS
6110
. In this chapter, the method of IS
6110
DNA fingerprinting is explained in such a way that it can be easily duplicated by molecular epidemiologists and will give reproducible results.
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