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Tn611 Transposon Mutagenesis in Mycobacterium smegmatis Using a Temperature-Sensitive Delivery System

 Last Update: 2021-02-22
 Source: Internet
 Author: User

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Mobile generic elements are discrete
DNA
fragments encoding their own transposltlon functions, which enable them to move from one site in the DNA to another, independently of host-recombination functions (such as RecA). Recombination by transposition occurs without homology between the sequences of the transposon and the target sequence (
1
). Moreover, most of these elements do not exhibit a strong specificity for the target-site sequence, thus allowing these elements to be widely used for mutagenesis in bacteria (
2
).

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