-
Storage of Mycobacterial Strains
Time of Update: 2021-02-20
The storage and maintenance of mycobacterial reference strains and clinical isolates are important parts of good laboratory practice in a mycobacterial laboratory.
The storage of reference and control strains facilitates a reliable control on intra- and inter-test reproducibility ( 1 , 12 ).
-
An Array Biosensor for Detection of Bacterial and Toxic Contaminants of Foods
Time of Update: 2021-02-20
Sandwich fluoroimmunoassays are performed on the surface of a patterned microscope slide that acts as an optical waveguide.
Fluorescence from immunocomplexes formed on the slide surface is excited using the evanescent field, an electromagnetic component of light, and the pattern of fluorescence is imaged using a charge-coupled device camera.
-
30 Cases of Peptic Ulcer Treated by Dele
Time of Update: 2021-02-20
2 results 29 cases of symptoms in the experimental group disappeared, and X-ray and gastroscopic review of the original ulcer healed with 95% efficacy.
In the control group, only 18 cases of symptoms disappeared, ulcers healed, and the effect was 60%.
-
Analysis of Cytomegalovirus Gene Expression by Transfection
Time of Update: 2021-02-20
Human cytomegalovirus (HCMV), a human herpesvirus, is the leading viral cause of birth defects ( 1 , 2 ).
HCMV has a severely restricted host range in culture.
-
Pulsed Field Gel Electrophoresis for Mycobacteria
Time of Update: 2021-02-20
The introduction of pulsed field gel electrophoresis (PFGE) was a major breakthrough in the field of genome research ( 1 ), allowing the analysis of whole procaryotic and eucaryotic chromosomes. This
-
Antigenic Typing of Field Isolates of Plasmodium by DNA Techniques
Time of Update: 2021-02-20
The frequency of the major mero- zoite surface antigen 1 (MSA-1) allelic forms in parasite strains iso- lated directly from patients varies dramatically from those found in in vitro cultured strains ( 6 , 7 ).
-
Analysis of Mitochondrial Gene Expression
Time of Update: 2021-02-20
Dictyostelium provides a well-established model system for the study of mitochondrial biology and disease.
Here we describe the methods deployed to study mitochondrial gene transcription and RNA processing inDictyostelium .
-
Characterization of Mycoplasmas by RAPD Fingerprinting
Time of Update: 2021-02-20
The diagnosis and typing of microorganisms of human and veterinary significance are vitally important in the rapid and effective treatment of infectious diseases.
Diagnostic microbiology laboratories are continuously updating methods in an effort to provide highly accurate, inexpensive, and rapid results for clinicians and research scientists.
-
Transformation of Brucella Species with Suicide and Broad Host-Range Plasmids
Time of Update: 2021-02-20
Thus, the ability to transformBrucella with suicide plasmids (to introduce transposons or cause allelic exchanges) or replicating plasmids (to perform complementation studies) is paramount in implementing molecular approaches to unravel the genetic basis forBrucella virulence determinants.
-
Assessment of Multidrug Efflux Assemblies by Surface Plasmon Resonance
Time of Update: 2021-02-20
Here we describe the application of this method to study interactions of membrane proteins involved in multidrug efflux inEscherichia coli.
The SPR approach provides means to overcome some of these problems in studies of protein assemblies that function in the context of two membranes.
-
Characterization of Specialized mtDNA Glycosylases
Time of Update: 2021-02-20
Mitochondria are cytoplasmic organelles that generate cellular energy in the form of ATP by the process of oxidative phosphorylation.
These few polypeptides encoded by mtDNA are essential components of oxidative phosphorylation.
-
Studying Adaptation and Homeostatic Behaviors of Kinetic Networks by Using MATLAB
Time of Update: 2021-02-20
A particular interesting type of model relates to the so-called robust behavior where the homeostatic or perfect adaptation property is independent of the magnitude of the applied step-wise perturbation.
-
Extraction of RNA from Intracellular Mycobacterium tuberculosis: Methods, Considerations, and Applications
Time of Update: 2021-02-20
Pathogenicity inMycobacterium tuberculosis may be thought of as a multifactorial process with both pathogen and host-response effector molecules contributing to the process of infection, leading eit
-
Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)
Time of Update: 2021-02-20
The application of molecular biology approaches holds the advantage of including culture-difficult and as-yet-uncultivated phylotypes in the profiles, providing a more comprehensive picture of the microbial community.
-
Poxviruses
Time of Update: 2021-02-20
Poxviruses are large, double-strandedDNA viruses that replicate in the cytoplasm of infected cells. The familyPoxviridae is divided into two subfamilies, theEntomopoxvirinae of insects and theChor
-
Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease
Time of Update: 2021-02-20
Celiac disease is diagnosed by the demonstration of an altered intestinal mucosa in a jejunal biopsy specimen ( 3 ).
Serum IgA antibodies against endomysial antibodies (EMAs) are especially considered to be sensitive and highly specific markers for celiac disease ( 8 )( 9 ).
-
Transcriptomic Analyses of Saliva
Time of Update: 2021-02-20
In this chapter, we will overview the development of sensitive and robust microarray and multiplex quantitative reverse transcriptase- PCR assays for the discovery and validation of mRNA biomarkers in human saliva.
-
Genomic DNA Digestion and Ribotyping
Time of Update: 2021-02-20
Such studies generally mvolve characterization to the genus and species level and provide an identification for the organism.
The practice of obtaining these patterns (or profiles) for epidemiologic purposes is the subject of the present chapter, although the methods can also be applied to questions regarding identification at higher taxonomic levels.
-
Engineering Escherichia coli for Canthaxanthin and Astaxanthin Biosynthesis
Time of Update: 2021-02-20
The carotenoid synthesis operons are assembled from genes isolated from carotenogenic bacterial sources.
Here, we describe methods to assemble carotenoid biosynthesis operons from carotenogenic gene clusters isolated from different bacterial sources and evaluate canthaxanthin or astaxanthin production inE.
-
Assay of mtDNA Polymerase from Human Tissues
Time of Update: 2021-02-20
MitochondrialDNA (mtDNA) replication is a complex process involving over 20 proteins organized along the inner mitochondrial membrane as a multienzyme complex called the mtDNA replisome, or replication factory (1 –3 ).
Figure 1 illustrates some of the protein components that participate in mitochondrial DNA replication.
