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The storage and maintenance of mycobacterial reference strains and clinical isolates are important parts of good laboratory practice in a mycobacterial laboratory. The storage of reference and control strains facilitates a reliable control on intra- and inter-test reproducibility (
1
,
12
). Furthermore, to study various aspects of the epidemiology of tuberculosis it is important to be able to study serial isolates of individual patients (
13
) or of patients associated with particular place or time factors (
14
).