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Poxviruses are large, double-stranded
DNA
viruses that replicate in the cytoplasm of infected cells. The family
Poxviridae is
divided into two subfamilies, the
Entomopoxvirinae
of insects and the
Chordopoxvirinae
of vertebrates; the latter consists of eight genera and several unclassified viruses, The genus
Orthopoxvirus
comprises morphologically and antigenically closely related viruses, including the now eradicated variola (smallpox) virus and several pathogens of veterinary and zoonotic importance.