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Premixed virus rapid quantitative detection reagents are coming!
Time of Update: 2021-08-09
With immediate effect, Takara Bio has started to sell Premix Real-Time PCR reagents [One Step PrimeScript III RT-qPCR Mix] (hereafter, this product) suitable for rapid virus detection and gene expression analysis .
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Multiplex PCR reagent with fast reaction speed & superior performance!
Time of Update: 2021-08-09
Among the several products compared, the cycle time of 20-fold PCR using SuperPlex Premix is the shortest, which saves a lot of time compared with competing products (see the table below for details) and the superior reaction performance as shown in the first experiment .
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Three Arrows Started-Thermo Fisher's three new QuantStudio real-time PCR instruments are on the market
Time of Update: 2021-08-09
In the future, QuantStudio1 and QuantStudio 3 IVD&5 IVD real-time fluorescent quantitative PCR instruments will uphold Thermo Fisher's consistent excellent quality and lean services, and work with Chinese users to serve clinical testing and help scientific research breakthroughs .
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Experts talk about the application of digital PCR in liquid biopsy[Innovative Skills]
Time of Update: 2021-08-09
Recently, Geoffrey Oxnard, a thoracic oncologist at the Dana-Farber Cancer Institute in the United States, accepted an interview with SelectScience and introduced the application of droplet digital PCR technology in liquid biopsy .
Oxnard used the QX100™ droplet digital PCR system to develop a ddPCR test .
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Document Express Naica Digital PCR Technology for Detection of PIK3CA Mutations in Plasma
Time of Update: 2021-08-09
Although data has been widely reported in eBC, no studies have focused on the molecular characterization and clinical outcomes of patients with PIK3CA mutant metastatic breast cancer (mBC) .
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The latest generation of digital PCR (cdPCR) landed in China [New product recommendation]
Time of Update: 2021-08-09
Welcome to request detailed information of Naica Crystal Digital PCR systemAbout Cycloud Deep Blue Cloud Deep Blue Cloud Biotechnology (Cycloudbio) is committed to providing you with new life science research instruments and analytical products as well as optimized overall application solutions.
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Real-time quantification, are you really doing it right?
Time of Update: 2021-08-09
The RACE test before real-time quantitative PCR is mainly for ① to determine the full-length sequence of the functional gene in the experimental sample; ② more critically, to determine whether the target gene has alternative splicing (also known as alternative splicing), variable The number of splicing and the corresponding sequence .
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Black Hole Quencher® (BHQ) black hole quencher combined with full-band fluorescent dyes,
Time of Update: 2021-08-09
Here is a perfect solution with BHQ!In fact, Biosearch's fluorescent dyes are far more than these, and they have truly achieved full-band coverage!qPCR and multiple qPCR primer and probe design utility In addition to high-efficiency fluorescent quenching dyes and various >https:// ) With the fluorophore spectrum selection tool ( http://qpcrdesign.
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Document Express Naica Digital PCR Detection of Cytokines
Time of Update: 2021-08-09
Recently, the Prion Laboratory of the Institute of Viral Disease Control and Prevention of the Chinese Center for Disease Control and Prevention published "IP10, KC and M-CSF is Remarkably Increased in the Brains from the Various Strains of Experimental Mice Infected with Different Scrapie Agents" in Virologica Sinica .
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Experimental design Drop-off digital PCR method two: gene mutation and gene deletion calculation method
Time of Update: 2021-08-09
Use Crystal Miner analysis software to quantitatively analyze Drop-off results step by stepThe wild-type concentration Cwt (double positive droplets) can be calculated directly by the ratio of Pwt (regardless of whether the third channel is positive or negative), and the concentration of drop-off mutation Cmut (mutant allele) can be calculated by Pmut .
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Chinese scientists developed an improved version of multiple displacement amplification method [Innovative Skills]
Time of Update: 2021-08-09
The combined use of MDA and next-generation sequencing (NGS) has become the gold standard method for genome research .
The authors concluded that they developed a simple and reliable solution to eliminate template-independent amplification in MDA .
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Direct amplification PCR-tissue/blood/plant sample preparation in 5 minutes
Time of Update: 2021-08-09
Loading dyes are included, which can be directly used for subsequent gel electrophoresis;In addition, the NucleoType Mouse PCR Direct Amplification Kit also provides a more time-saving direct PCR process, that is, the mouse tissue sample does not need to be lysed at room temperature, and the PCR reaction is directly performed .
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Will digital PCR change the monitoring and treatment of leukemia?
Time of Update: 2021-08-09
According to Patel, since the introduction of digital PCR in Bio-Rad in 2011, multiple research teams have developed alternative methods for measuring the level of BCR-ABL transcripts using ddPCR .
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Skip RNA purification and launch the new Cells-to-CT kit [New product recommendation]
Time of Update: 2021-08-09
The new Fast Advanced Cells-to-CT kit uses real-time fluorescent quantitative RT-PCR to directly detect relative gene expression in cultured cell samples, allowing you to skip tedious and time-consuming RNA purification steps .
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"0" PCR, the nemesis of the wrong InDel is here!
Time of Update: 2021-08-09
In the library construction stage of high-throughput sequencing, the library template is amplified thousands of times by PCR to achieve the library volume on the computer to complete the subsequent determination of the genomic DNA sequence .
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New product release QIAcuity integrated nanochip digital PCR system
Time of Update: 2021-08-09
QIAGEN's new digital PCR system QIAcuity based on integrated nanochips simplifies the experimental process to be as simple and fast as qPCR experiments .
The instrument automatically presses the sample into the nanopores of the microfluidic chip and seals each hole independently .
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The French company Stilla Technologies released the development project of the high-throughput digital PCR system Naica HT and the 6-color digital detection system
Time of Update: 2021-08-09
Caroline Charky, Vice President of Commercial Operations of Stilla Company, released the high-throughput digital PCR system Naica HT and the new Opal chip at the SLAS Conference (Laboratory Automation and Screening Conference) in February 2019, which can detect ≥240 samples per day.
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Naica™ crystal automatic microdrop chip digital PCR system
Time of Update: 2021-08-09
The Naica™ crystal automatic microdrop chip digital PCR instrument system is the next generation of absolute nucleic acid quantitative technology developed by the French company Stilla Technologies .
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Clontech new product awards help SNP genotyping
Time of Update: 2021-08-09
Among them, the restriction enzyme method based on electrophoresis detection (PCR-RFLP) is one of the more classic methods, but the premise of the application of this technology is that the site of the SNP must contain the recognition of the restriction enzyme.
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How to determine the blank detection limit LOB of digital PCR?
Time of Update: 2021-08-09
LOB (Limit of Blank) is an important parameter to judge the detection ability of digital PCR technology .
When quantifying nucleic acids, the cutoff of false positives is critical to the stability of dPCR detection .
