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Skip RNA purification and launch the new Cells-to-CT kit [New product recommendation]

 Last Update: 2021-08-09
 Source: Internet
 Author: User

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Real-time quantitative RT-PCR is a classic method to study gene expression levels in cells

That year, Thermo Fisher's Cells-to-CT kit was widely praised by researchers as soon as it went on the market

With the Fast Advanced Cells-to-CT kit, you will experience a faster reverse transcription reaction, and you can use the fully optimized qPCR master mix to improve the sensitivity of rare transcript detection

The new Fast Advanced Cells-to-CT kit uses real-time fluorescent quantitative RT-PCR to directly detect relative gene expression in cultured cell samples, allowing you to skip tedious and time-consuming RNA purification steps

This kit uses a unique method to lyse cultured cells while removing genomic DNA (gDNA) and maintaining RNA integrity.

The Cells-to-CT stop solution can irreversibly terminate the lysis reaction, allowing most samples to enter the downstream RT and qPCR reactions, greatly improving the sensitivity of rare target detection

Thermo Fisher’s vice president of sample preparation, Ole Dahlberg, said: “The latest product in our Cells-to-CT kit product portfolio provides researchers with the fastest workflow to date to detect high abundance or Rare transcripts

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