Direct amplification PCR-tissue/blood/plant sample preparation in 5 minutes

Direct PCR (Direct PCR) uses unpurified samples for PCR amplification without the need for nucleic acid purification steps, which brings unprecedented convenience to DNA amplification and saves a lot of time in spring

To do direct PCR, two types of necessary reagents (sample lysate and PCR master mix) and a component that remove or inhibit inhibitors that affect DNA amplification in the sample are indispensable

The difference in the composition of different brands of lysate makes the lysing ability different

PCR master mix recommends the use of hot-start DNA polymerase to enhance specific amplification and increase the amplification ability

In addition, samples that are more difficult to handle, such as plants and blood, contain more inhibitor components that can affect the structure and activity of DNA polymerase and hinder the combination of template with DNA polymerase and primers, making PCR amplification difficult

German MACHEREY-NAGEL (MN) has a direct amplification PCR detection kit for mice, blood, plant leaves and seeds

The overall concept of the product is:

1.

NucleoType Mouse PCR

Designed for rapid genotyping experiments in mice, suitable for clipped mouse tails (1 mm), ear size inlay sampling mouse ears (1 mm Ø ), mouse blood (1 µL), fur (eg.

Features

In addition, the NucleoType Mouse PCR Direct Amplification Kit also provides a more time-saving direct PCR process, that is, the mouse tissue sample does not need to be lysed at room temperature, and the PCR reaction is directly performed

NucleoType Blood PCR-Simplify the blood typing test process

Used for rapid genetic analysis and detection of blood spots in whole blood (treated with anticoagulants such as EDTA, citrate or heparin) and blood cards

Features

NucleoType Plant PCR

It is suitable for rapid and simple genotyping analysis of plant leaves

NucleoType Seed PCR

Used for rapid genotyping experiments of plant seeds or hard plant materials

Gene Co.