Real-time quantification, are you really doing it right?

When studying mRNA function, the most routine operation is to use real-time quantitative PCR to analyze the expression of functional genes

Because scientific researchers have overlooked an important test link-RACE

Rapid amplification of cDNA ends (RACE) is an effective method to rapidly amplify the 5'and 3'ends of cDNA from low-abundance transcripts based on PCR

A large number of functional genes encode different proteins through alternative splicing, and different alternative splicing of the same gene can even perform different or even completely opposite functions

The above figure is an example.

Before real-time quantitative PCR, only through the RACE technology to obtain the alternative splicing sequence information of the target sequence, that is, whether there is alternative splicing, and design the corresponding real-time quantitative PCR detection primers for the different regions of different alternative splicing bodies, in order to obtain the corresponding The expression of alternative spliceosome to determine the effect of experimental treatment on gene expression

So, how do we choose RACE kits?

We recommend Tiosbio® SUPERSWITCH RACE cDNA Synthesis Kit

1.

The Tiosbio® SUPERSWITCH RACE cDNA synthesis kit has excellent performance and can achieve efficient and stable full-length gene cloning results, making it easier for researchers to obtain alternative splicing of functional genes

Let us choose the correct functional gene research operation, let us choose Tiosbio® SUPERSWITCH RACE cDNA synthesis kit

Request details of Tiosbio SUPERSWITCH RACE cDNA synthesis kit now