Document Express Naica Digital PCR Detection of Cytokines

Recently, the Prion Laboratory of the Institute of Viral Disease Control and Prevention of the Chinese Center for Disease Control and Prevention published "IP10, KC and M-CSF is Remarkably Increased in the Brains from the Various Strains of Experimental Mice Infected with Different Scrapie Agents" in Virologica Sinica

Document content

Prion Disease

Scrape is caused by prions in subviruses, and is a long-term progressive, exhausting and lethal neurological disease

Laboratory introduction

The prion ward laboratory is affiliated to the Institute for Viral Disease Control and Prevention, which is an independent legal entity under the Chinese Center for Disease Control and Prevention

The main research direction of the prion ward laboratory: to provide scientific basis for the formulation of regulations, standards, technical guidelines and plans for the prevention and control of prion diseases; to provide technical support for human and animal prion disease epidemics and public health emergencies Participate in and guide the investigation and handling of local related epidemics and public health emergencies; organize the national human prion disease monitoring work, collect, analyze, interpret, report and feed back monitoring data, evaluate and improve the monitoring system; coordinate with the national mad cow disease Monitor network work; carry out monitoring and detection technology research and technical reserve, provide technical guidance for prion disease prevention and control work at all levels of disease control institutions; develop prion etiology, epidemiology, genetics, neurobiology, and individual biology Research on the pathogenesis of prion diseases such as science and other scientific research, carry out corresponding biotechnological research such as diagnosis, prevention and treatment, carry out research on other degenerative diseases of the central nervous system; establish and improve a reference laboratory for human prion diseases, Carry out the preservation of prion disease-related virus species and biological samples and related biosafety research; carry out prion disease-related postgraduate education and training and professional technical training for disease control personnel across the country, carry out relevant international cooperation and exchanges, and participate in prions Disease prevention, control and research; undertake other tasks assigned by superiors

Summary

In the central nervous system of patients with prion diseases, the activation of inflammatory cells and the up-regulation of some cytokines are often observed

In this paper, the absolute number of IP10 transcripts in the brain tissue of infected mice was detected using Naica digital PCR.

As shown in the figure below, real-time fluorescent quantitative PCR and digital PCR are used to compare and analyze the transcription levels of IP10, KC and M-CSF in various mouse models of scrapie infection

A: Relative to the transcription level of a single β-actin, the transcription level of IP10, KC and M-CSF-specific mRNA was determined

You can copy this link address to read the full text:

Naica Digital PCR Platform

The French Stilla Technologies Naica digital PCR platform is a technical tool to achieve absolute quantification of DNA/RNA.

☑ Only one step of sample addition is required, no standards and standard curves are required to be a true absolute quantitative system

Journal Introduction

Virologica Sinica is the head of the Chinese Academy of Sciences, an academic journal co-sponsored by the Wuhan Institute of Virology of the Chinese Academy of Sciences and the Chinese Society for Microbiology.

Beijing Shenlanyun Biotechnology Co.