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Biocom reports Multiple displacement amplification (MDA) is an isothermal amplification method that is often used for whole genome amplification
One of the corresponding authors of this article is associate professor Xiaofeng Fan of St.
Multiple displacement amplification is widely used for genome or transcriptome amplification
However, MDA also has a long-standing problem, which is template-independent amplification (TIA), especially when amplifying ultra-low concentrations of templates
Therefore, people use various methods to eliminate junk DNA, but these methods are not widely used
For this reason, the research team at St.
The researchers collected 30 serum samples, extracted RNA from 0.
The results of the study showed that compared with conventional MDA, the quantifiable DNA amplification in the negative control was significantly reduced after tdMDA treatment, which was reflected in the reduction of unalignable sequences in Illumina sequencing (7 ± 10.
The authors concluded that they developed a simple and reliable solution to eliminate template-independent amplification in MDA
In-depth understanding of the REPLI-g multiple displacement amplification product known as the genome copier
Original Search
Template-dependent multiple displacement amplification for profiling human circulating RNA
BioTechniques, Vol.
