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PCR machine, did you really choose the right one?
Time of Update: 2021-08-10
The aforementioned thermal overshoot of the module requires very rich experience, and an accurate algorithm can make samples of different volumes reach a stable set point temperature without overshoot and related negative effects .
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Instead of PCR, the application of RPA in cancer research
Time of Update: 2021-08-10
RPA for cancer mutation detectionThe Singapore A*STAR research team published in Lab on a Chip magazine last June , a new technology that can quickly detect single point mutations in cancer, Isothermal Solid Phase Amplification / Detection ( ISAD ) .
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PCR alternative technology, common problems of RPA primers and probes
Time of Update: 2021-08-10
Adding different enzymes and probes to this basic system can realize diversified RPA applications, such as RNA amplification detection and real-time RPA detection .
Do RPA primers need special purification?When performing primer screening, purification is generally not required .
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Digital PCR technology for precise detection of viral RNA mutations [Innovative Tips]
Time of Update: 2021-08-10
Bio-Tong reported that researchers in the United States and Canada found that Bio-Rad’s droplet digital PCR (ddPCR) technology can achieve higher accuracy, sensitivity, and accuracy compared with traditional reverse transcription quantitative PCR (RT-qPCR) technology.
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Convenient SNP typing detection reagent [new product recommendation]
Time of Update: 2021-08-10
The LightMix® kit uses a hybridization probe combined with a melting curve analysis genotyping method, which can complete qPCR amplification and typing detection in the same reaction tube at one time.
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Detection of host cell residual DNA, ddPCR has many benefits [new product recommendation]
Time of Update: 2021-08-10
Compared with real-time quantitative PCR technology, the advantage of using droplet digital PCR (ddPCR) technology and premix is that the DNA purification step can be skipped and the residual DNA of the host cell can be directly determined .
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PCR alternative technology, the troubleshooting of RPA
Time of Update: 2021-08-10
(Extended reading: A revolution in nucleic acid detection, which can replace the sharp technology of PCR )What temperature does the RPA reaction need to be carried out?Standard TwistAmp® operating temperature of the kit at 37 ° C - 42 ° C between .
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What are the challenges for quantitative PCR standardization?
Time of Update: 2021-08-10
Six years ago, scientists proposed a set of unified evaluation standards—MIQE guidelines for various problems in real-time quantitative PCR experiments .
"However, although more and more researchers are using dPCR in their experiments, few adopt the digital MIQE guidelines .
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How to distinguish the copy number variation of heterogeneous samples [Innovative Tips]
Time of Update: 2021-08-10
They recently released an application guide that combines cell sorting and digital PCR to distinguish copy number variations in heterogeneous samples .
However, if it is a cell mixture, such as HEK 293-GFP and HTB-70 cells mixed in a ratio of 50:50, the copy number is 4.
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Why not skip RNA purification for gene expression analysis?
Time of Update: 2021-08-10
To this end, Bio-Rad recently launched a new rapid cell lysis kit that allows researchers to obtain RT-qPCR data directly from cultured cells without the need for separate RNA purification .
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QX200 automatic droplet digital PCR system
Time of Update: 2021-08-10
In order to better simplify the operation process of droplet digital PCR and realize the concept of walk-away, Bio-Rad specially introduced the corresponding fully automatic model (QX200 Auto DG Droplet Digital PCR System) based on the QX200 droplet digital PCR system.
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Experimental design One of the Drop-off digital PCR methods: detection of gene mutations and gene deletions
Time of Update: 2021-08-09
In the presence of the wild-type allele, both the Drop-off probe and the Reference probe will hybridize to the target, generating a double positive signal (Figure 1B, blue cluster) .
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The latest generation of digital PCR (cdPCR) landed in China [New product recommendation]
Time of Update: 2021-08-09
Welcome to request detailed information of Naica Crystal Digital PCR systemAbout Cycloud Deep Blue Cloud Deep Blue Cloud Biotechnology (Cycloudbio) is committed to providing you with new life science research instruments and analytical products as well as optimized overall application solutions.
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Experts talk about the application of digital PCR in liquid biopsy[Innovative Skills]
Time of Update: 2021-08-09
Recently, Geoffrey Oxnard, a thoracic oncologist at the Dana-Farber Cancer Institute in the United States, accepted an interview with SelectScience and introduced the application of droplet digital PCR technology in liquid biopsy .
Oxnard used the QX100™ droplet digital PCR system to develop a ddPCR test .
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Three Arrows Started-Thermo Fisher's three new QuantStudio real-time PCR instruments are on the market
Time of Update: 2021-08-09
In the future, QuantStudio1 and QuantStudio 3 IVD&5 IVD real-time fluorescent quantitative PCR instruments will uphold Thermo Fisher's consistent excellent quality and lean services, and work with Chinese users to serve clinical testing and help scientific research breakthroughs .
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Multiplex PCR reagent with fast reaction speed & superior performance!
Time of Update: 2021-08-09
Among the several products compared, the cycle time of 20-fold PCR using SuperPlex Premix is the shortest, which saves a lot of time compared with competing products (see the table below for details) and the superior reaction performance as shown in the first experiment .
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Establishment of Crystal Digital PCR Three-channel Detection Method for HER-2 Gene Copy Number Variation
Time of Update: 2021-08-09
In this study, DNA extracted from 7 breast cancer patient samples provided by the French Gustave Roussy Institute was used for detection, and the results obtained by Crystal digital PCR were compared with those obtained by standard diagnostic procedures .
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Real-time quantification, are you really doing it right?
Time of Update: 2021-08-09
In addition to short transcripts that are related to human immune function, long transcripts and four moderate-length transcripts expressed in coronary artery endothelial or vascular smooth muscle cells are all involved in CAD .
First, researchers should use RACE technology or three-generation full-length transcriptome sequencing technology to determine whether the target gene has alternative splicing .
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Premixed virus rapid quantitative detection reagents are coming!
Time of Update: 2021-08-09
With immediate effect, Takara Bio has started to sell Premix Real-Time PCR reagents [One Step PrimeScript III RT-qPCR Mix] (hereafter, this product) suitable for rapid virus detection and gene expression analysis .
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Document Express Naica Digital PCR Technology for Detection of PIK3CA Mutations in Plasma
Time of Update: 2021-08-09
Although data has been widely reported in eBC, no studies have focused on the molecular characterization and clinical outcomes of patients with PIK3CA mutant metastatic breast cancer (mBC) .
