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Two unique new products of quantitative PCR instrument [New product recommendation]
Time of Update: 2021-08-10
In September last year, Bibby Scientific acquired PCRmax, and then launched the Eco 48 quantitative PCR instrument .
The temperature of the entire heating plate must be uniform to ensure that all samples are processed the same .
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Experts from the Academy of Agricultural Sciences use RPA to quickly detect genetically modified crops
Time of Update: 2021-08-10
(Extended reading: Nature topic: Thirty years of genetically modified crops )A few days ago, the research team of the Institute of Biotechnology of the Chinese Academy of Agricultural Sciences realized the rapid detection of genetically modified crops on the basis of RPA .
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What you need to know about PCR, qPCR, dPCR
Time of Update: 2021-08-10
(For more detailed information, see: SYBR Real-time Quantitative PCR Optimization Guide )Although both qPCR and dPCR can quantify nucleic acids in a sample, they have an important difference .
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When the quantitative PCR instrument meets the cloud computing platform [new product recommendation]
Time of Update: 2021-08-10
In addition to the benefits of sharing anytime and anywhere, using the cloud computing platform, the analysis speed is also increased by 10 times than before, so that more data can be analyzed and clues can be obtained faster .
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The latest masterpiece of Applied Biosystems, ProFlex is on the market!
Time of Update: 2021-08-10
Since Applied Biosystems designed and manufactured the world's first PCR instrument in 1987, it has continued to innovate over the past 30 years, leading a series of innovative design revolutions. Th
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Plant Direct PCR Technology
Time of Update: 2021-08-10
Just cut about 5mg of plant leaves and put them into solution L, incubate at 95°C for 10 minutes, without grinding with liquid nitrogen, and then add an equal volume of solution I, you can directly perform PCR, which is convenient and quick, and it is a scientific researcher in the field of plants.
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PCR alternative technology, primer design of RPA strategy
Time of Update: 2021-08-10
For the 3 ' end of the 3 nucleotides, the guanine and cytosine help stabilize polymerase binding, can improve the performance of the amplification primers .
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Smaller, faster and stronger?
Time of Update: 2021-08-10
The portable qPCR from Ahram, South Korea, uses a unique upgraded temperature detection principle, breaking through the limit of traditional detection speed, and achieving an ultra-high detection spee
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It is said to be the smallest PCR detector in the world [new product recommendation]
Time of Update: 2021-08-10
Spartan Cube uses polymerase chain reaction (PCR) technology to carry out different DNA tests in as little as 30 minutes .
Lem did not specify the price of Cube and test kits .
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New product focus in Jena, Germany (3): Fashion PCR instrument Biometra TOne
Time of Update: 2021-08-10
Biometra TOne, as the latest member of the red PCR family in Jena, Germany, continues the advantages of the Biometra PCR instrument, such as fast, accurate, stable, and quiet, and incorporates some innovative designs, such as a 7-inch full-color touch screen and an intuitive software interface .
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Replace PCR, RPA to detect deadly viruses such as Ebola
Time of Update: 2021-08-10
(Reference: Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis )RPA detects ten deadly threats such as EbolaThe comprehensive panel for detecting infectious diseases is helpful for on-site molecular diagnosis in resource-poor areas, and it is also very helpful for biological defense work .
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Instead of PCR, RPA plays an important role in HIV testing
Time of Update: 2021-08-10
A study in September of this year showed that when the room temperature is between 31°C-43°C , the RPA reaction can be successfully incubated for 20 minutes , and 10 copies of HIV-1 proviral DNA can be detected .
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PrimePCR™ Assays and Panels keep you away from the trouble of designing primers and probes
Time of Update: 2021-08-10
For PrimePCR™ Assays, each pair of primers for human, mouse, and rat genomes has been verified by qPCR experiments, and the verification report can be downloaded online, which complies with MIQE international guidelines to ensure data accuracy .
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Digital PCR to find diagnostic markers of breast cancer [Innovative Tips]
Time of Update: 2021-08-10
In this study, researchers at the University of Ferrara in Italy used Bio-Rad's QX200 droplet digital PCR (ddPCR) system to determine the absolute levels of key miRNA molecules in plasma and serum samples from a variety of cancer patients .
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Takara's new quantitative PCR product debut
Time of Update: 2021-08-10
Takara’s newly launched CellAmp™ Direct RT-qPCR Kit does not require RNA extraction from various animal cells, and can perform 2-step Real Time PCR with a simple operation .
5 hours from template preparation to reverse transcription reaction, gene expression analysis and other operations .
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A KRAS mutation detection method superior to sequencing [Innovative Tips]
Time of Update: 2021-08-10
Recently, an international team led by Chinese scientists published an article in the Journal of Experimental & Clinical Cancer Research, claiming that the peptide nucleic acid clamp PCR (PNA-PCR) technology is more sensitive than Sanger sequencing in detecting KRAS mutations .
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A revolution in nucleic acid detection, which can replace the sharp technology of PCR
Time of Update: 2021-08-10
Not only can you perform end-point detection on the amplified products, you can also monitor the amplification process in real time, and even read the results through a test strip (lateral flow chromatography test strip LFD ) .
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Regarding digital PCR, please keep these dry goods [experience comment]
Time of Update: 2021-08-10
According to the Poisson distribution principle and the proportion of positive droplets, the absolute number of target molecules in the sample can be determined .
For Bio-Rad's droplet digital PCR system (ddPCR), approximately 1-100,000 copies of DNA target molecules are required per well .
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RPA technology test water library preparation for next-generation sequencing
Time of Update: 2021-08-10
(Extended reading: A revolution in nucleic acid detection, which can replace the sharp technology of PCR ) This attempt shows that the optimized RPA technology will be expected to be used in sample preparation for next-generation sequencing in the future .
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Inhibitor-tolerant qPCR reagents are freshly launched [new product recommendation]
Time of Update: 2021-08-10
BioTone reported that Bio-Rad recently announced the launch of a PCR inhibitor-resistant qPCR master mix-SsoAdvanced™ Universal Inhibitor-Tolerant SYBR® Green Supermix, which can obtain high-quality qPCR data from the most challenging samples .
