Replace PCR, RPA to detect deadly viruses such as Ebola

Biocom reports: Recombinase Polymerase Amplification ( RPA ) is known as a nucleic acid detection technology that can replace PCR (developed by the British company TwistDx Inc )

RPA to detect Plasmodium

Nucleic acid amplification is currently the most sensitive specific detection method for malaria parasite ( P.

In a study published in " Malaria Journal " in March this year, researchers combined RPA basic reaction, TwistAmp nfo probe and lateral flow chromatography to achieve high-sensitivity and rapid detection of the Plasmodium 18S rRNA gene

The researchers tested the sensitivity and specificity of this method with different malaria parasites

RPA detects ten deadly threats such as Ebola

The comprehensive panel for detecting infectious diseases is helpful for on-site molecular diagnosis in resource-poor areas, and it is also very helpful for biological defense work

A study in " Journal of Clinical Microbiology " last April developed an RPA detection panel for Francisella tularensis , Yersinia pestis , and Bacillus anthracis .

RPA to detect coronavirus

The Middle East respiratory syndrome coronavirus ( MERS-CoV), which was first identified in the Middle East in 2012 , is a new type of coronavirus .

Currently, MERS-CoV detection mainly relies on real - time RT-PCR , which requires collecting patient samples and then going to a specialized laboratory for testing

A study published in " PLOS Currents : Outbreaks " last December developed a portable RT-RPA device that can identify MERS-CoV in 3-7 minutes .

In addition, researchers around the world have recently used RPA technology to detect many other viruses

Biological Communication Editor: Ye Yu