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Six years ago, scientists proposed a set of unified evaluation standards—MIQE guidelines for various problems in real-time quantitative PCR experiments
When people with zero foundation are preparing to carry out quantitative PCR, most of them will invite teachers and sisters
Fortunately, a group of researchers has developed a roadmap for dPCR experiments, called the digital MIQE guide
However, although more and more researchers are using dPCR in their experiments, few adopt the digital MIQE guidelines
This is consistent with an earlier survey by Nature Methods, which showed that about nine of the ten articles failed to provide the minimum required experimental data, and therefore may contain misleading results and conclusions
For this situation, the senior author of the digital MIQE paper, Professor Stephen Bustin of Ruskin University in Anglia, UK believes that the implementation of MIQE guidelines requires the participation of journal editors
However, Professor Bustin also said that instrument suppliers have become active promoters of MIQE guidelines
These tools can give researchers a clearer understanding of good PCR practices
I want to learn more about Bio-Rad's digital PCR system
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GUIDING OUR PCR EXPERIMENTS
BioTechniques, Vol.
