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How to determine the blank detection limit LOB of digital PCR?

 Last Update: 2021-08-09
 Source: Internet
 Author: User

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Digital PCR technology is a new nucleic acid detection and quantification technology

LOB (the limit of blank) is a statistical experimental parameter

Why calculate LOB in digital PCR?

In digital PCR (dPCR), multiple factors can cause false positives

The 95% confidence LOB is shown in the following table:

in:

R: number of repetitions, generally ≧30

When μ=0 (that is, false positives will never occur), then LOB (95%)=0.

For more information about LOB values, please log in to the statistical tools of Gene-π Digital PCR School (Digital PCR School Quick Entry).

Beijing Shenlanyun Biotechnology Co.

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