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Scir pus yagara
Time of Update: 2020-11-12
Widely distributed in northeast China, north China, southwest and even the Yangtze River basin provinces and regions and Taiwan.
Perennian herbs, root-like stems long and stout, top raw spout tubers.
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A Method to Discover Phased siRNA Loci
Time of Update: 2020-11-12
Short, interfering RNAs (siRNAs) arise from the processing of long double-stranded RNA (dsRNA) by Dicer enzymes.
Dicers generate siRNA duplexes by successive hydrolysis of both strands of the dsRNA phosphodiester backbone at positions determined by measuring 21–24 nucleotides from an exposed dsRNA terminus.
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Cryopreservation of Zygotic Embryonic Axes and Somatic Embryos of European Chestnut
Time of Update: 2020-11-12
For zygotic embryonic axes, the highest post-thaw survival and plantlet recovery rates are obtained by desiccation in a laminar flow hood to 20–25% moisture content, followed by direct immersion in liquid nitrogen.
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Characterizing Transgene Inheritance
Time of Update: 2020-11-12
The analysis of transgene inheritance is an important step in the molecular and genetic characterization of transgenes.
In this manuscript, two approaches to characterize the inheritance of transgenes are described.
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Use of Robust-Long Serial Analysis of Gene Expression to Identify Novel Fungal and Plant Genes Involved in Host-Pathogen Interactions
Time of Update: 2020-11-12
Recently, we developed an improved LongSAGE method called robust-long serial analysis of gene expression (RL-SAGE) for deep transcriptome analysis of fungal and plant genomes.
Our results demonstrate that RL-SAGE is an effective approach for large-scale identification of expressed genes in fungal and plant genomes.
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Egyptian Clover
Time of Update: 2020-11-12
Seeds yellow or tawny, 1,000 weight 2-3g.
The average daily temperature in spring is 17 degrees C, so you can grow: low temperatures in winter are not suitable for planting in areas below -6 degrees Celsius.
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Nanyang fir
Time of Update: 2020-11-12
, Nanyang fir belongs to Nanyang fir, evergreen trees, 40-60 meters high, chest diameter 1.2 meters.
Chile's southern fir, also known as South American fir, 30-50 meters high, chest diameter 1-1.5 meters.
The seeds are about 150, pear-like, native to the coastal areas of Oceania.
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Plant Transgenesis
Time of Update: 2020-11-12
Epigenetic effects such as gene silencing and variable expression are unintended consequences of plant transformation, a problem that is present in the transformation of all plant species.
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Measurements of Cytosolic Ion Concentrations in Live Cells
Time of Update: 2020-11-12
Here, we describe a series of methods suitable for the measurement of cytosolic ion concentrations in living plant cells using ion selective dyes.
We describe procedures for the use of SBFI for the measurement of Na + in live cells.
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Isolation of Sequences Flanking Ac Insertion Sites by Ac Casting
Time of Update: 2020-11-12
Or, a researcher may have a small sequence surrounding theAc / Ds insertion site and needs to obtain additional flanking genomic sequences.
Here we describe the principle ofAc casting, and show how it can be applied to isolateAc macrotransposon insertion sites.
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Cinderella.
Time of Update: 2020-11-12
Cinderella plant height 15-30 cm, sloppy, leaf-to-birth, egg-shaped or long-ovoid, jagged edge, leaves with rough hair, touch with sand.
Leaves long oval, wrinkled, leafy green-brown or copper-red, with a metallic luster, leaf back fuchsia.
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Mechanical Isolation of Bundle Sheath Cell Strands and Thylakoids from Leaves of C4 Grasses
Time of Update: 2020-11-12
We have developed procedures for mechanical disruption of leaf tissues in order to isolate metabolically active bundle sheath strand cells and thylakoids practically free from cross-contamination coming from mesophyll cells.
Using mechanical disruption we have shown the supramolecular organization of the dimeric LHCII-PSII in BS thylakoids of maize.
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In situ Analysis of Gene Expression in Plants
Time of Update: 2020-11-12
In the post-genomic era, it is necessary to adapt methods for gene expression and functional analyses to more high-throughput levels of processing.
We have adapted this technique as a high-throughput means of gene expression analysis on wax-embedded plant tissues and also on whole-mount tissues.
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Studying Arabidopsis Chloroplast Structural Organisation Using Transmission Electron Microscopy
Time of Update: 2020-11-12
Chloroplasts, as well as other, non-photosynthetic types of plastid, are characteristic structures within plant cells.
Transmission electron microscopy (TEM), therefore, represents a powerful technique for monitoring the effects of various changing parameters or treatments on the development and differentiation of these important organelles.
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Quantifying 13C-labeling in Free Sugars and Starch by GC-MS
Time of Update: 2020-11-12
We describe an approach to extract13 C-labeled sugars (glucose, fructose, maltose, sucrose,myo -inositol as well as glucose from starch) from plant tissues and to analyze their isotopomer distribution by gas chromatography–mass spectrometry (GC-MS).
The method is illustrated by sugar isotopomer analysis of13 C-labeled rapeseed embryos.
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Insertion of Introns: A Strategy to Facilitate Assembly of Infectious Full Length Clones
Time of Update: 2020-11-12
It has been speculated that unintended transcription and translation result in expression of proteins that are toxic to the bacteria.
If the clone is constructed for transcription in vivo, interrupting the virus sequence with an intron can solve the toxicity problem.
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Leguminosae
Time of Update: 2020-11-12
K (5) C 5 A (9) 1, (5) (5), (10), 10,∞ G 1:1 Gemal leaf plants, usually divided into shoal-shredded yako, yunshi (or Sumu) yako and butterfly yako.
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Bridging the Gene-to-Function Knowledge Gap Through Functional Genomics
Time of Update: 2020-11-12
The explosion of genomics data has led to a significant knowledge gap, with thousands of genes identified having no known function.
The following chapter describes the available forward and reverse genetics strategies, which can assist researchers in assigning functions to novel genes.
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Culture and Time-Lapse Tracking of Barley Microspore-Derived Embryos
Time of Update: 2020-11-12
Barley microspore embryogenesis represents an attractive system to study stress-induced cell differentiation and is a valuable tool for efficient plant breeding.
While liquid culture systems allow the generation of large amounts of embryos for molecular analysis, solid culture systems are the ultimate tool for probing embryo development.
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Agrobacterium-Mediated Transformation: Rice Transformation
Time of Update: 2020-11-12
In agricultural plant biotechnology, combination of non-phytopathogenic strain ofAgrobacterium tumefaciens with modified T-DNA andvir-genes in a binary vector system is the most widely utilized system for genetic improvement in diverse plant species and for gene function validation.
Here we have described a highly efficientA.
