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Non-invasive Flux Measurements Using Microsensors: Theory, Limitations, and Systems
Time of Update: 2020-11-11
Knowledge of the fluxes of ions and neutral molecules across the outer membrane or boundary of living tissues and cells is an important strand of applied molecular biology.
Two systems (MIFE™ and SIET) have been developed and have become widely used to implement this technique, and they are commercially available.
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葱（Allium fistulosum）
Time of Update: 2020-11-11
Flower stem cylindrical, hollow, below the middle of the puffy, narrow to the top, the lower part by the leaves, the total membrane quality, 2 cracked.
Umbrella-shaped flower sequence, multi-flower, flower handle base without small slivers, flower white near-egg-shaped, the first end gradually tip.
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Global Quantitative Proteomics Using Spectral Counting: An Inexpensive Experimental and Bioinformatics Workflow for Deep Proteome Coverage
Time of Update: 2020-11-10
There have been many recent reviews on quantitative proteomic approaches, discussing the strengths and limitations of each.
Unfortunately, there are few detailed methodological descriptions of any one of these quantitative approaches.
Here we present a detailed bioinformatics workflow for one of the simplest, most pervasive quantitative approach—spectral counting.
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Sweet potatoes (Ipomoea batatas)
Time of Update: 2020-11-10
Commonly known as sweet potatoes, melons and so on.
Red purple or white, flower crown flower shape, the umbrella flower sequence, sometimes single, total flower terrier length; fruit, seeds have 4 , hairless.
Native to the tropical region of South America.
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Analytical Kinetic Modeling: A Practical Procedure
Time of Update: 2020-11-10
This chapter describes a practical procedure to dissect metabolic systems, simplify them, and use or derive enzyme rate equations in order to build a mathematical model of a metabolic system and run simulations.
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Phoebe.
Time of Update: 2020-11-10
Garden use: this species of tree trunk tall straight, the crown is majestic, suitable for the shade tree and evening tree use, in the production area gardens and temples common cultivation.
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Isolation of Chloroplast Proteins from Arabidopsis thaliana for Proteome Analysis
Time of Update: 2020-11-10
This chapter describes a simple protocol for large-scale chloroplast purification for proteome analysis. The protocol has not been tested for protein import activity but is optimized for chloroplast
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Proteomic Analysis of Thylakoid Membranes
Time of Update: 2020-11-10
These peptide fragments were separated by MALDI-TOF, and the isotopic masses were blasted to a MASCOT server to obtain the protein sequence.
Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF).
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Elimination of Marker Genes From Transgenic Plants Using MAT Vector Systems
Time of Update: 2020-11-10
We have developed an efficient system (Multi-Auto-Transformation [MAT] vectors) for the removal of marker genes and to increase the regeneration frequency of transgenic crops without using antibiotic selection, reducing their possible environmental impact.
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Identification of Cyclic Nucleotide Gated Channels Using Regular Expressions
Time of Update: 2020-11-10
The methods detail how to employ regular expressions of conserved amino acids in functional domains of annotated CNGCs and together with Web tools such as PHI-BLAST and ScanProsite to identify novel candidate CNGCs in species includingPhyscomitrella patens .
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The Study of Transgene Copy Number and Organization
Time of Update: 2020-11-10
This chapter details the routine use of quantitative real-time polymerase chain reaction to determine transgene copy number in putative transgenic events.
Implementation of the TaqMan transgene copy assay permits the efficient utilization of limited resources and space to develop a highly efficient transgenic event production pipeline.
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Heart skin (carpel)
Time of Update: 2020-11-10
the unit that forms the female of the bee plant. It is a perverted leaf with reproductive function. It is covered with raw embryo beads, and the beads are wrapped in them. The healing place when th
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Phenotyping Jasmonate Regulation of Root Growth
Time of Update: 2020-11-10
Root architecture is a complex and highly plastic feature of higher plants.
Here, we describe a fast, noninvasive, and semiautomatic method to monitor root architectural responses to environmental stimuli using plant tissue culture and the software toolEZ-Rhizo .
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Nandina domestica
Time of Update: 2020-11-10
Cultivation of a brief history and flower culture : Nantian bamboo branches as bamboo, feather leaves carried out and beautiful, autumn and winter season turned red, unusually gorgeous, spiked fruit on the red fruit, bright and dazzling.
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Split-Transgene Expression in Wheat
Time of Update: 2020-11-10
This chapter describes the methodology for producing herbicide-resistant and pollen-sterile wheat plants by the intein-mediated assembly of inactive precursor protein fragments (proteintrans -splicing).
We suggest the design of intein-containing vectors for split-transgene expression.
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Transposon Display: A Versatile Method for Transposon Tagging
Time of Update: 2020-11-10
In species containing high copy numbers of transposable elements combined with a high transposition rate, forward cloning can be quite challenging and requires specific high-resolution methods.
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Grapes are a warmbed for rooting
Time of Update: 2020-11-10
During the rooting to keep sand or sawdust moist, and pay attention to control the bed surface temperature, during the day will cover the hotbed of plastic film to open, the use of early spring cold air to reduce the bed surface temperature, to prevent premature germination of bud eyes.
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Studying Starch Content and Sedimentation of Amyloplast Statoliths in Arabidopsis Roots
Time of Update: 2020-11-10
Amyloplasts, organelles responsible for the synthesis and storage of starch, are of critical importance to gravitropism in higher plants.
Second, we discuss a method for measuring amyloplast sedimentation in the dynamic environment of Arabidopsis root columella cells, which is thought to provide a directional cue to a reoriented plant.
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Mother (common anemarrhena)
Time of Update: 2020-11-10
Barcasting line is 25cm, covered with 2cm of soil, and the seeding volume is 7.5-12kg per hectare.
Planted according to the plant distance of 15-20cm, the mulch pressure watering seedling period needs to be loose soil weeding.
According to 15cm plant distance seedlings, long-term appropriate fertilizer.
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Microspore Embryogenesis and In Vitro Pollen Maturation in Tobacco
Time of Update: 2020-11-10
In a rich medium, they can develop into mature pollen grains that are fertile on pollination in vivo ( 1 , 2 ).
Their development closely resembles pollen formed in vivo, whereas the in vitro culture conditions simulate the changing environment a microspore/pollen grain experiences inside the anther.
