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Atractylodes.
Time of Update: 2020-11-11
White art is a special product of Zhejiang Province of China, produced in Lin'an County in the sub-region called "in (in) art".
Its roots are thick, like fists, as medicinal herbs, to the Tianmian mountain area produced the most precious.
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Transcriptomics on Small Samples
Time of Update: 2020-11-11
Among the challenges arising when extracting RNA from individual plant cells are: the isolation of pure cell populations; the small yield of material when isolating specific cell types, and ensuring an accurate representation of the transcriptome from each cell type after amplification of RNA.
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Constructing Haustorium-Specific cDNA Libraries from Rust Fungi
Time of Update: 2020-11-11
The haustorium is a distinguishing feature of biotrophic plant pathogens.
Despite their clear importance in biotrophy, they have been difficult to study due to the close association of biotrophic pathogens with their host and the inability to produce haustoria in vitro.
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Laser Microdissection of PlantFungus Interaction Sites and Isolation of RNA for Downstream Expression Profiling
Time of Update: 2020-11-11
The molecular mechanisms that mediate the intimate interaction of an adapted obligate biotroph, such as the powdery mildewGolovinomyces orontii , on its host plant are spatially and temporally distinct.
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Analyzing Small and Long RNAs in Plant Development Using Non-radioactive In Situ Hybridization
Time of Update: 2020-11-11
We are interested in understanding the role of various non-coding RNAs (including miRNAs) in the regulation of root and nodule development in legumes, which are agriculturally important crops.
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Immunogold Labeling of Resin-Embedded Electron Microscopical Sections
Time of Update: 2020-11-11
Knowledge about the spatio-temporal distribution patterns of proteins and other molecules of the cell is essential for understanding their function.
A widely used technique is immunolabeling which uses specific antibodies to reveal the distribution of molecular components at various structural levels.
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Inoculation and Virulence Assay for Bacterial Blight and Bacterial Leaf Streak of Rice
Time of Update: 2020-11-11
Despite being very closely related, the pathogens colonize different tissues and cause distinct diseases.
Here we describe protocols for Xoo and Xoc inoculation and disease scoring methods that are appropriate to their different modes of infection.
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Arabidopsis thaliana Membrane Lipid Molecular Species and Their Mass Spectral Analysis
Time of Update: 2020-11-11
Herein, current approaches to electrospray ionization mass spectrometry-based analyses of membrane lipid molecular species found inArabidopsis thaliana are summarized. Additionally, the identities o
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Fluorescence-based Electrophoretic Mobility Shift Assay in the Analysis of DNA-binding Proteins
Time of Update: 2020-11-11
Here we describe a workflow for purification, characterisation and identification of DNA-binding proteins.
This involves a crude enrichment of nucleic acid binding proteins by heparin–Sepharose chromatography and the characterisation of fractions using overlapping fluorescence-labelled DNA probes spanning the promoter region of interest.
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Insect Vector Transmission Assays
Time of Update: 2020-11-11
Following acquisition from the infected source plant, there is a latent period before the vector can transmit, so transmission assays consist of three basic steps: acquisition, latency, and inoculation.
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Non-invasive Flux Measurements Using Microsensors: Theory, Limitations, and Systems
Time of Update: 2020-11-11
Knowledge of the fluxes of ions and neutral molecules across the outer membrane or boundary of living tissues and cells is an important strand of applied molecular biology.
Two systems (MIFE™ and SIET) have been developed and have become widely used to implement this technique, and they are commercially available.
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Sorghum (Sorghum bicolor L.)
Time of Update: 2020-11-11
Agrobacterium co-transformation vectors, one T- DNA containing the selectable marker gene and another T-DNA containing the trait gene(s), were also introduced in sorghum transformation for eliminating the selectable marker gene in the resulting transgenic plants.
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Nitric Oxide Methods in Seed Biology
Time of Update: 2020-11-11
Experiments with this biologically important gas require special provisions because NO in aerobic environments is readily converted into other oxides of nitrogen.
Methods for detecting NO using NO-reactive fluorescent probes, and an apparatus incorporating an oxidizer column are also described.
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Protein Extraction from Xylem and Phloem Sap
Time of Update: 2020-11-11
It is well known that phloem and xylem vessels transport small nutrient molecules over long distances in higher plants.
This chapter outlines how proteins can be obtained and purified from xylem and phloem saps to perform subsequent proteomic analyses.
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Root Transformation by Agrobacterium tumefaciens
Time of Update: 2020-11-11
Genetic transformation and clonal propagation are techniques that play an important role in the identification and characterization of plant genes and their products. The Joint efforts to develop Ara
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Golden Leaf Wenshulan
Time of Update: 2020-11-11
Golden leaf Wenshulan, new leaf green, old leaf golden yellow, bright colors, like sufficient bright light.
White line Wenshulan leaves have white vertical lines, leaf color fresh and vulgar, sexual preference bright indirect light, not resistant to strong direct sunlight.
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In Vitro Rejuvenation of Woody Species
Time of Update: 2020-11-11
Juvenility and phase change in woody plant species exert profound impacts on plant morphology and the ability of explants to be successfully propagated in vitro.
Physiological maturity, that is the ability to reproduce sexually, is reached by many woody species only after many years of juvenile growth.
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葱(Allium fistulosum)
Time of Update: 2020-11-11
Flower stem cylindrical, hollow, below the middle of the puffy, narrow to the top, the lower part by the leaves, the total membrane quality, 2 cracked.
Umbrella-shaped flower sequence, multi-flower, flower handle base without small slivers, flower white near-egg-shaped, the first end gradually tip.
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Nan.
Time of Update: 2020-11-11
Nanmu wood fine, beautiful texture, not cracking, moisture and non-corrosion, fire, aromatic and shiny, is extremely valuable building materials.
Famous such as Zinan, also known as Zijinnan, 20 meters high, tree-shaped beauty, can be used as a landscape tree.
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Masan
Time of Update: 2020-11-11
The 1st section of the Geminal Leaf Plant Gang Magnolia Yagang. Shrubs, with prisms. Single leaf, born or rotating, no leaves, flowers of both sexes or mono sex, neat, single born in the leaf or in