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Visualizing and Characterizing In Vivo DNA-Binding Events and Direct Target Genes of Plant Transcription Factors
Time of Update: 2020-11-13
These new types of data require the use of advanced bioinformatic tools in order to understand the molecular mechanisms of functional specificity and target gene regulation by transcription factors.
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Plant Tissue Culture
Time of Update: 2020-11-13
plants< a href" > organization< > Plant Tissue Culture is an isolated part of a plant that is grown using sterile culture, i.e. a way to grow or develop plant cells or tissues isolated from the n
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Multigene Phylogenetic Analyses to Delimit New Species in Fungal Plant Pathogens
Time of Update: 2020-11-12
Does one have a new species when a strain differs by a few base pairs when compared to reference sequences from taxonomically well-characterized species that do not differ morphologically from this new strain?
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Capturing Insect Vectors of Phytoplasmas
Time of Update: 2020-11-12
While populations can be monitored by a number of passive techniques in the field, the capture of live insects is necessary for manipulation and study.
A number of physical methods for capturing these insects already exist, but more innovative traps equipped with infochemical lures for species-specific monitoring and mass trapping are being developed.
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Visual Mapping of Cell Wall Biosynthesis
Time of Update: 2020-11-12
We have also successfully utilized bimolecular fluorescence complementation (BiFC) for in situ visualization of protein–protein interactions of pectin biosynthetic enzymes and for the determination of their membrane topology in the Golgi apparatus.
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Cryogenic Technologies for the Long-Term Storage of Citrus Germplasm
Time of Update: 2020-11-12
Like majority of the woody fruit plants,Citrus germplasm is conserved mainly as field collections in clonal orchards.
However, such a traditional approach presents several difficulties, among which are the high cost, manual labor, and extensive land required to maintain the collections, as well as the necessity of a careful protection of plants from diseases and extreme environmental conditions.
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In Vitro Propagation of Olive (Olea europaea L.) by Nodal Segmentation of Elongated Shoots
Time of Update: 2020-11-12
Olive ( Olea europaea L.), long-living, ever-green fruit tree of the Old World, has been part of a traditional landscape in the Mediterranean area for centuries.
However today, the progress in micropropagation technology has made available the complete protocols for several Mediterranean cultivars.
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Sorghum.
Time of Update: 2020-11-12
Planting in the northeast of China is quite common, because its stems are tall can be Tibetan, sorghum is also commonly known as "green yarn account", during the Japanese invasion period, the anti-Japanese guerrillas often infested, there was a period when the Japanese invading forces once banned farmers from planting sorghum.
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Coryllusheterophylla
Time of Update: 2020-11-12
Male and female, male flowers into order, sagging, female flowers single or cluster born in the last year of the side branches.
Born among the jungles of the mountain slopes.
Positive trees, like well-drained soil, but drought-resistant, barren, even in limestone mountains can grow.
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Biolistic-Mediated Transformation Protocols for Maize and Pearl Millet Using Pre-Cultured Immature Zygotic Embryos and Embryogenic Tissue
Time of Update: 2020-11-12
Biolistic-mediated transformation and the production of transgenic plantlets via somatic embryogenesis of two maize strains viz.
Maize and pearl millet cultures were regenerated via somatic embryogenesis as they are bipolar structures that shoot and root simultaneously.
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Dodder Transmission of Phytoplasmas
Time of Update: 2020-11-12
Transmission of phytoplasmas from naturally infected plant host species using the parasitic plantCuscuta spp.
Restriction fragment length polymorphism analyses of PCR fragments can also be used to confirm the identity of the phytoplasmas from donor and recipient plants.
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Immunolocalization of Proteins in Plants
Time of Update: 2020-11-12
Moreover, recombinant protein techniques are applicable only to a rather limited set of model plants.
The immunolocalization protocols presented here can be used to display protein localization patterns in different tissues of various plant species.
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Artificial MicroRNAs for Specific Gene Silencing in Rice
Time of Update: 2020-11-12
For the plant breeder, gene silencing by artificial miRNAs will certainly accelerate gene discovery, because it allows targeting of all genes in a mapping interval, independent of the genetic background.
This chapter describes the generation and application of amiRNAs as a gene silencing tool in rice.
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Construction of Gene Interruptions and Gene Deletions in the Cyanobacterium Synechocystis sp. Strain PCC 6803
Time of Update: 2020-11-12
A series of protocols are presented for the storage, growth, transformation, and characterization of wild type and mutant strains ofSynechocystis sp.
These protocols include the isolation of genomicDNA and the strategies required for the construction of specific gene interruptions or deletions in this organism.
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Yang, Hebei Province
Time of Update: 2020-11-12
Leaf eggs round or nearly round, long 3-8cm, edge with loose or irregular missing teeth, blunt or short tip, base round or near-cut shape, the back of the young leaf is thick fluff, and then gradually fall off; Distribution: mainly distributed in north and northwest China provinces, vertical distribution of Shanxi at an altitude of 800-1500m , Gansu in 1000-2000m .
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Assaying Protein S-Acylation in Plants
Time of Update: 2020-11-12
Heterotrimeric G-proteins and G PCR s are particularly well studied S-acylated proteins, and fast, cheap, reliable methods are required for the analysis of S-acylation states of these proteins.
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Chromatin Charting: Global Mapping of Epigenetic Effects
Time of Update: 2020-11-12
Using this collection of plants, one can begin to map transgene position effects as well as global epigenetic control in response to developmental or externally applied cues.
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Mechanical Isolation of Bundle Sheath Cell Strands and Thylakoids from Leaves of C4 Grasses
Time of Update: 2020-11-12
We have developed procedures for mechanical disruption of leaf tissues in order to isolate metabolically active bundle sheath strand cells and thylakoids practically free from cross-contamination coming from mesophyll cells.
Using mechanical disruption we have shown the supramolecular organization of the dimeric LHCII-PSII in BS thylakoids of maize.
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Cinderella.
Time of Update: 2020-11-12
Cinderella plant height 15-30 cm, sloppy, leaf-to-birth, egg-shaped or long-ovoid, jagged edge, leaves with rough hair, touch with sand.
Leaves long oval, wrinkled, leafy green-brown or copper-red, with a metallic luster, leaf back fuchsia.
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In situ Analysis of Gene Expression in Plants
Time of Update: 2020-11-12
In the post-genomic era, it is necessary to adapt methods for gene expression and functional analyses to more high-throughput levels of processing.
We have adapted this technique as a high-throughput means of gene expression analysis on wax-embedded plant tissues and also on whole-mount tissues.
