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Dodder Transmission of Phytoplasmas

 Last Update: 2020-11-12
 Source: Internet
 Author: User

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Transmission of phytoplasmas from naturally infected plant host species using the parasitic plant
Cuscuta
spp. (dodder) to
Catharanthus roseus
(Madagascar periwinkle) is an effective way to maintain a wide range of phytoplasmas for further research. Here, we describe transmission via dodder from an infected medicinal plant
Rehmannia glutinosa
var.
purpurea
and from a symptomatic redcurrant plant (
Ribes
spp.) to
C
.
roseus
indicator plants using a “stable bridges” method. In both cases, typical symptoms of phytoplasma disease on periwinkle plants were obtained: virescent flowers with an increased number of axillary shoots and smaller leaves after transmission from
R
.
glutinosa
, and greening petals (virescence) after transmission from
Ribes
spp. Phytoplasmas could be detected in donor and recipient plants by electron microscopy and by polymerase chain reaction (
PCR
) assays using universal phytoplasma primer pairs. Restriction fragment length polymorphism analyses of PCR fragments can also be used to confirm the identity of the phytoplasmas from donor and recipient plants.

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