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In situ Analysis of Gene Expression in Plants

 Last Update: 2020-11-12
 Source: Internet
 Author: User

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quantitative analysis methods

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In the post-genomic era, it is necessary to adapt methods for gene expression and functional analyses to more high-throughput levels of processing. mRNA in situ hybridization (ISH) remains a powerful tool for obtaining information regarding a gene’s temporal and spatial expression pattern and can therefore be used as a starting point to define the function of a gene or a whole set of genes. We have deconstructed ‘traditional’ ISH techniques described for a range of organisms and developed protocols for ISH that adapt and integrate a degree of automation to standardized and shortened protocols. We have adapted this technique as a high-throughput means of gene expression analysis on wax-embedded plant tissues and also on whole-mount tissues. We have used wax-embedded wheat grains and
Arabidopsis
floral meristems and whole-mount Arabidopsis roots as test systems and show that it is capable of highly parallel processing.

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