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Anther Culture of Chili Pepper (Capsicum spp.)
Time of Update: 2020-11-13
Chili pepper ( Capsicum spp.) is a very important horticultural crop around the world and is especially important for Mexicans because of its impact in the culture and the cuisine.
In this chapter, a protocol for efficient recovery of chili pepper haploid plants from in vitro cultured anthers is described.
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Pack Peony Dicentra Spectabilis
Time of Update: 2020-11-13
Total flower sequence top, sagging, petals 4 pieces, the outer two petals of a larger joint into a heart-shaped sac, pink, first end to the two sides of the back volume inside two slender, the first end protruding white.
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Agrobacterium tumefaciens Chemotaxis Protocols
Time of Update: 2020-11-13
Responses to nanomolar amounts of phenolic wound exudates are chiefly determined by the Ti-plasmid encoded virulence genesvir A andvir G ( 5 , 6 ).
Thus, the attraction to sugars can partly explain the organism’s prevalence in the rhizosphere ( 7 ) and the highly sensitive response to phenolics, may help to guideA.
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Highly Efficient Transformation Protocol for Plum (Prunus domestica L.)
Time of Update: 2020-11-13
The hypocotyl slices are infected in anAgrobacterium tumefaciens suspension and then cocultivated for 3 days in shoot regeneration � MS basal medium supplemented with 9 μM 2,4-dichlorophenoxyacetic acid.
Transformed shoots are rooted in � MS basal medium supplemented with 5 μM NAA and 40 mg/L kanamycin.
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Pyrethrum cinerariifolium
Time of Update: 2020-11-13
Head sequence contains pyrethroid acetylene, B (Pyrethrin I.
II.) and chrysanthemum acetylene, B (cinerin I., II.), pyrethroid beetle has the strongest insecticidal capacity, 10 times pyrethroid b.
In addition to chrysanthemum instability, easy to hydrolysis, insecticidal powder should not survive.
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Purple Spike (shrubby falseindigo)
Time of Update: 2020-11-13
Each breeding method directly nursery transplant and seeding, generally to spring sowing as a good, such as sufficient soil moisture, spring sowing and summer sowing can be.
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A hundred riding big chestnut trees
Time of Update: 2020-11-13
, the queen of the ancient Arab kings, once led a hundred riders to Mount Etna to play, suddenly it rained heavily, hundreds of riders rushed to the big chestnut tree to shelter from the rain.
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High-Throughput TILLING for Functional Genomics
Time of Update: 2020-11-13
Here, we describe the basic methodology for high-throughput TILLING.
Gene segments are amplified using fluorescently tagged primers, and products are denatured and reannealed to form heteroduplexes between the mutated sequence and its wild-type counterpart.
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Daisy (Dendranthema morifolium)
Time of Update: 2020-11-13
Head-shaped flower sequence single or several sets born at the top of stems, diameter 2.5 to 20 cm, depending on the variety, very different; Origin of China and Japan.
Large chrysanthemums use yellow flower artemisinin, large seed artemisinin, etc.
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Analysis of Starch Metabolism in Chloroplasts
Time of Update: 2020-11-13
These methods can be used to investigate transitory starch metabolism in Arabidopsis, where they can be applied in combination with genetics and systems-level approaches to yield new insight into the control of carbon allocation generally, and starch metabolism specifically.
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Plant Mitochondrial Proteomics
Time of Update: 2020-11-13
Here we outline a mitochondrial isolation protocol that can be applied to a range of plant tissues, and detail methods of assessing the quality and purity of the resultant sample, including calculations of respiratory control ratio, marker enzyme assays, differential in-gel electrophoresis, and quantitative gel-free mass spectrometry.
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Peanut Transformation
Time of Update: 2020-11-13
Peanut or groundnut ( Arachis hypogaea L.) is a member of the legume family, originated in South America, and today is largely cultivated in many tropical and subtropical areas worldwide.
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Yang, Xinjiang
Time of Update: 2020-11-13
Short branches of the leaves near the round table, there is a lack of cut-shaped thick teeth, the back of the young dense white fluff, and then gradually fall off nearly hairless; Xinjiang Yang is mainly distributed in Xinjiang, especially in the southern Xinjiang region more, in recent years, China's northern provinces have many introduced species, growth is good.
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Plant Tissue Culture
Time of Update: 2020-11-13
plants< a href" > organization< > Plant Tissue Culture is an isolated part of a plant that is grown using sterile culture, i.e. a way to grow or develop plant cells or tissues isolated from the n
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Gene Tagging with Engineered Ds Elements in Maize
Time of Update: 2020-11-13
We describe here protocols for isolating genes in maize usingDissociation (Ds ) transposons marked with a green fluorescent protein ( GFP ) transgene.
Enough transgenic lines are being generated to cover most of the maize genome, allowing the targeted tagging of most genes from aDs-launching platform located nearby.
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Australian nuts (macadamia nut)
Time of Update: 2020-11-13
the most suitable growth temperature is 15-30 degrees C, above 30 degrees C growth is seriously hindered, 10 degrees C stop growing, the elderly tree can withstand short-term -4 to -5.6 degrees C low temperature, in the annual precipitation of 1300-3000 mm, dry and wet season obvious tropical and warm frost-free subtropical areas cultivated optimally.
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DNA Bar-Coding for Phytoplasma Identification
Time of Update: 2020-11-13
DNA barcoding is an identification method based on comparison of a short DNA sequence with known sequences from a database.
A DNA barcoding tool has been developed for phytoplasma identification.
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Peach
Time of Update: 2020-11-13
Grafting, the use of cutting method, with two years of raw peach seedlings as birch.
Press the strips, which are carried out in late May after flowering, and cut off the potted plants in autumn.
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Visualizing and Characterizing In Vivo DNA-Binding Events and Direct Target Genes of Plant Transcription Factors
Time of Update: 2020-11-13
These new types of data require the use of advanced bioinformatic tools in order to understand the molecular mechanisms of functional specificity and target gene regulation by transcription factors.
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Transformation of Rice via PEG-Mediated DNA Uptake into Protoplasts
Time of Update: 2020-11-13
However, there is still no convincing evidence of sustained divisions in protoplasts isolated from leaves or shoots of any cereal In contrast, protoplasts isolated from embryogenic suspension cultures could be induced to divide in culture ( 4 ).
