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Gene Tagging with Engineered Ds Elements in Maize

 Last Update: 2020-11-13
 Source: Internet
 Author: User

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We describe here protocols for isolating genes in maize using
Dissociation (Ds
) transposons marked with a green fluorescent protein (
GFP
) transgene. The introduced marker enables the phenotypic scoring of the nonautonomous element and the anchoring of unique primers on the element to facilitate the isolation of the adjacent
DNA
by
PCR
. Transposons such as
Ds
transpose preferentially to sites closely linked to the
Ds
-launching platform. Based on this transposition behavior, a genetic resource is being created to mobilize a modified
Ds
element from different starting sites in the genome. Enough transgenic lines are being generated to cover most of the maize genome, allowing the targeted tagging of most genes from a
Ds-launching
platform located nearby.

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