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Isolation of Sequences Flanking Ac Insertion Sites by Ac Casting

 Last Update: 2020-11-12
 Source: Internet
 Author: User

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Localizing
Ac
insertions is a fundamental task in studying
Ac
-induced mutation and chromosomal rearrangements involving
Ac
elements. Researchers may sometimes be faced with the situation in which the sequence flanking one side of an
Ac
/
Ds
element is known, but the other flank is unknown. Or, a researcher may have a small sequence surrounding the
Ac
/
Ds
insertion site and needs to obtain additional flanking genomic sequences. One way to rapidly clone unknown
Ac
/
Ds
flanking sequences is via a
PCR
-based method termed
Ac
casting. This approach utilizes the somatic transposition activity of
Ac
during plant development, and provides an efficient means for short-range genome walking. Here we describe the principle of
Ac
casting, and show how it can be applied to isolate
Ac
macrotransposon insertion sites.

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