-
Transformation of Rape with Agrobacterium tumeficiens-Based Vectors
Time of Update: 2020-11-20
Agrobacterium tumefaciens , a soil bacterium that causes crown gall disease on a wide range of dicotyledonous plants, has been used most widely as a vehicle for gene transfer into plants ( 1 , 2 ).
-
Putting the Plant Metabolic Network Pathway Databases to Work: Going Offline to Gain New Capabilities
Time of Update: 2020-11-20
Metabolic databases such as The Plant Metabolic Network/MetaCyc and KEGG PATHWAY are publicly accessible resources providing organism-specific information on reactions and metabolites.
This chapter describes accessing and using AraCyc and its underlying Pathway Tools software.
-
Transformation of Tomato
Time of Update: 2020-11-20
Since 1986, when Beachy and coworkers ( 1 ) first published a protection of transgenic tobacco plants expressing the tobacco mosaic virus (TMV) coat protein (CP) against TMV infection, transgenic plants have become an important tool in plant virology.
Coat protein transgenic plants have been used successfully to obtain protection against many different plant viruses ( 2 ).
-
Indica Rice (Oryza sativa, BR29 and IR64)
Time of Update: 2020-11-20
To satisfy the growing demand of the ever-increasing population, more sustained production of indica-type rice is needed.
Gene transfer technology with genes from other sources can be used to make rice plants resistant or tolerant to insect pests, diseases, and different environmental stresses.
-
Arabidopsis Proteomics: A Simple and Standardizable Workflow for Quantitative Proteome Characterization
Time of Update: 2020-11-20
Furthermore, most Arabidopsis organs are susceptible to standard protein solubilization protocols making protein extraction relatively simple.
We report here a simple, standardizable protocol for the large-scale comparative quantitative proteome characterization of different Arabidopsis organs based on normalized spectral counting and suggest a statistical framework for data interpretation.
-
Phytohemagglutin/Lectin
Time of Update: 2020-11-20
Lectins are proteins in nature with molecular weight ranging from 60,000 to 100,000 Da. Many lectins contain covalently bound sugar moieties and are glycoprotein in nature. These are also called phyt
-
Collection of ball root flowers
Time of Update: 2020-11-20
Potted flowers, after the plant withered, cut off the stems and leaves, the original pot placed in the indoor moist shelter, the temperature remains at 3 degrees C-5 degrees C, you can hibernate.
-
Territories.
Time of Update: 2020-11-20
Subsequently, it was suggested that organisms should be divided into 3 boundaries, namely, plant, animal and primary biology, and later it was suggested that primary nuclear organisms should become independent of the original nuclear biological community, and organisms should be divided into 4 boundaries, and then fungus is also divided into one sector, the 5 sector, and in recent years, according to the characteristics of the virus, it has been classified as the 6th virus boundary of the organism.
-
Cloning of Stress-Responsive MicroRNAs and other Small RNAs from Plants
Time of Update: 2020-11-20
This chapter describes a detailed step-by-step protocol for cloning of small RNAs. Following 5ʹ and 3ʹ adapter ligation to the purified small RNAs, c DNA will be synthesized using reverse transcription, which will be further amplified using a polymerase chain reaction.
-
Immunoproteomics Analysis of Food Allergens
Time of Update: 2020-11-20
Immunoblot-coupled proteomics based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), namely, immunoproteomics, has been used for comprehensive identification of food allergens, becaus
-
Distinguishing Variable Phenotypes from Variegation Caused by Transposon Activities
Time of Update: 2020-11-20
Variable phenotypes are common in nature and in laboratory materials. Guidelines and illustrations are presented to help distinguish developmental, environmental, disease, and somatic recombination-g
-
cDNA-AFLP-Based Transcript Profiling for Genome-Wide Expression Analysis of Jasmonate-Treated Plants and Plant Cultures
Time of Update: 2020-11-20
Quantitative expression data are generated by gel-based visualization ofcDNA -AFLP fingerprints obtained by selectivePCR amplification of subsets of restriction fragments from a double-stranded cDNA template.
The differentially expressed genes can be identified by direct sequencing of re-amplified cDNA-AFLP tags purified from the gels.
-
In Vivo 1H-NMR Microimaging During Seed Imbibition, Germination, and Early Growth
Time of Update: 2020-11-19
We have shown that high-resolution1 H-nuclear magnetic resonance ( NMR ) microimaging can provide a wealth of information about the internal anatomy of plant seeds as small as 1 mm or even smaller.
-
Cotton (Gossypium)
Time of Update: 2020-11-19
(cotton bell) egg shape, 3.5 to 5 cm long, seed skin formed long cotton hair and gray-white cinder hair.
Cotton fiber is an excellent textile raw material, seed oil content of about 40%, oil for medicinal use of milking.
-
A New Temporary Immersion Bioreactor System for Micropropagation
Time of Update: 2020-11-19
A new type of bioreactor system for plant micropropagation is described that incorporates a number of features specifically designed to simplify its operation and reduce production costs.
-
Using Sequential Fluorescence and Genomic In Situ Hybridization (FISH and GISH) to Distinguish the A and C Genomes in Brassica napus
Time of Update: 2020-11-19
We have developed a sequential procedure with fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) that enables us to distinguish between the A and C genomes inBrassica
-
Three-Dimensional Acrylamide Fluorescence In Situ Hybridization for Plant Cells
Time of Update: 2020-11-19
In particular, optical sectioning microscopy of fluorescence in situ hybridization (FISH)-stained nuclei provides three-dimensional spatial information about the organization and distribution of specific sequences and chromosomal loci within the nucleus.
Several examples illustrate the versatility of this technique for the investigation of meiotic telomere dynamics in maize,Arabidopsis , and oat.
-
Quantifying Alternatively Spliced mRNA via Capillary Electrophoresis
Time of Update: 2020-11-19
BecauseRPS4 expression levels are very low and the pattern ofRPS4 splicing is complex, existing mRNA quantification methods were not adequate.
This method is especially suitable for quantification of alternative transcripts of low-expressed genes exhibiting complex splicing patterns.
-
Use of Statistics in Plant Biotechnology
Time of Update: 2020-11-19
Statistics and experimental design are important tools for the plant biotechnologist and should be used when planning and conducting experiments as well as during the analysis and interpretation of r
-
Studying Chloroplast Protein Interactions In Vitro: An Overview of the Available Methods
Time of Update: 2020-11-19
A large variety of methods are available, which range from qualitative assays allowing for screening for new interaction partners, and semiquantitative assays allowing for a rough description of the interaction between two partners, to quantitative assays that permit detailed determination of kinetic and thermodynamic parameters.
