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Using Sequential Fluorescence and Genomic In Situ Hybridization (FISH and GISH) to Distinguish the A and C Genomes in Brassica napus

 Last Update: 2020-11-19
 Source: Internet
 Author: User

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situ hybridization definition

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We have developed a sequential procedure with fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) that enables us to distinguish between the A and C genomes in
Brassica napus
and to identify certain individual chromosomes or chromosome groups within a genome. Our modified GISH technique uses a repetitive sequence in addition to the whole genome in the blocking
DNA
, and it is effective on meiotic and mitotic cells present in the anther material that we use.

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