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Rubber Tree (Hevea brasiliensis Muell. Arg)
Time of Update: 2020-11-18
We found that a suitable transformation protocol forHevea consists of a 3-d co-cultivation withAgrobacterium in the presence of 20 m M acetosyringone, 15 m M betaine HCl, and 11.55 m M proline followed by selection on medium containing 300 mg/L kanamycin.
Transformed calli surviving on medium containing 300 mg/L kanamycin showed a strong GUS-positive reaction.
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Transformation of Barley (Hordeum vulgare L.) by Agrobacterium tumefaciens Infection of In Vitro Cultured Ovules
Time of Update: 2020-11-18
Agrobacterium -mediated transformation of in vitro cultured barley ovules is an attractive alternative to well-established barley transformation methods of immature embryos.
The ovule culture system can be used for transformation with and without selection and has successfully been used to transform cultivars other than Golden Promise indicating minor genotype dependency.
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Tobacco Transformation
Time of Update: 2020-11-18
For researchers interested in unraveling the roles of specific genes in particular pathways of growth and development, the introduction into plants of foreign genes and gene promoters linked to reporter genes allows the detailed study of the temporal, spatial, and quantitative expression of plant genes and the activities of associated regulatory sequences.
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Tung.
Time of Update: 2020-11-18
, there are six kinds of oil in the world, with the origin of China's three-year and millennium slugs are the most common.
Millennium learning famous wood oil, cause and peel wrinkles, so also known as turtle back, meaning long life hundred years old.
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常青藤 Hedera nepalensis var.sinensis
Time of Update: 2020-11-18
Umbrella flower sequence, small flowers, white-green, slightly fragrant.
application value: The south is planted in front of buildings, for three-dimensional greening of fine plant materials, the north more potted plants.
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30 Cloning Genes of Arabidopsis thaliana by Chromosome Walking
Time of Update: 2020-11-18
Repeated rounds (or steps) of such screening would eventually yield an overlapping set of clones (contig) bridgmg the mitral RFLP marker and the gene of interest Arabidopsis thaliana is particularly suited for isolating specific genes by this approach because of the small size of the haploid content of its genome and the paucity of repetitiveDNA 2
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Activation Tagging Using the Maize En-I Transposon System for the Identification of Abiotic Stress Resistance Genes in Arabidopsis
Time of Update: 2020-11-18
An activation tag (ATag) library of 800 lines was generated in Arabidopsis ecotype Columbia using theEn-I ( Spm ) transposon system.
The ATag lines were used in a forward genetics strategy to identify novel genes that confer resistance/tolerance to abiotic stresses.
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Biolistic Transformation of Cotton Embryogenic Cell Suspension Cultures
Time of Update: 2020-11-18
Genetic transformation of cotton ( Gossypium hirsutum L.) is highly dependent on the ability to regenerate fertile plants from transgenic cells through somatic embryogenesis.
Here I describe a detailed procedure to transform cotton embryogenic cell suspension cultures by biolistic bombardment.
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桃（Prunus persica）
Time of Update: 2020-11-18
Native to China, wild peaches are found in Shaanxi, Gansu, Tibet and western Henan.
The origin of cultivation is very early, China's ancient book "Psalms" "Arya" and other books have been planted peaches, so at least 3000 cultivation history.
Fruit is a famous fruit, can be raw food or canned, peach.
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Assaying Effector Function in Planta Using Double-Barreled Particle Bombardment
Time of Update: 2020-11-18
The double-barreled gene gun device is an inexpensive and highly effective attachment that enables statistically significant data to be obtained with one-tenth the number of experimental replicates compared to conventional biolistic assays.
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Fragrant leaf geranium
Time of Update: 2020-11-18
The Soviet Union, Morocco and Algeria were also cultivated, but mainly pink leaf geraniums.
In the early 20th century, China introduced a small number of plants, as potted ornamental plants, after 1949 began to produce productive cultivation.
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Methods for Generation and Analysis of Fluorescent Protein-Tagged Maize Lines
Time of Update: 2020-11-18
Localization of tagged proteins using native regulatory elements has the advantage that it is less likely to generate artifactual results, and also reports tissue-specific expression patterns for the gene of interest.
This chapter describes methods to generate and characterize fluorescent protein-tagged maize lines driven by their native regulatory elements.
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The birch
Time of Update: 2020-11-18
in the dense forests of Shishan in guangxi, southeastern Yunnan and northern Vietnam, there is a very hard wood tree called birch.
Guangxi Longzhou County, a large birch, about 40 meters high, the trunk diameter of about 1 meter, age between 450-500 years old, this is probably the largest birch.
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Measurements of Viability Suitable for Plant Tissue Cultures
Time of Update: 2020-11-18
Viability is the capacity of a cell or organism to live, and the state of being viable is most commonly recognized by the growth of the studied tissues ( 1 - 3 ).
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Expression and Purification of Active Protein Kinases from Wheat Germ Extracts
Time of Update: 2020-11-18
In vitro functional studies of eukaryotic kinases are often constrained by the availability of pure and �enzymatically active kinase of interest.
Current wheat-germ-based cell-free in vitro translation systems present a plausible alternative for protein synthesis since majority of eukaryotic proteins could be obtained in their native folded form with general protocols.
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Survey of Natural and Transgenic Gene Markers Used to Monitor Transposon Activity
Time of Update: 2020-11-18
Marker genes have played a critical role in the discovery of plant transposable elements, our understanding of transposon biology, and the utility of transposable elements as tools in functional genomics.
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Fruit Organ Cultures
Time of Update: 2020-11-18
Typically, however, when an isolated portion of the fruit tissue is introduced into a sterile environment, it immediately loses structural integrity and degenerates into a rapidly dividing callus mass ( 2 ).
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RNA Extraction from Cereal Vegetative Tissue
Time of Update: 2020-11-18
In some instances, total RNA is all that is required; however most applications will require the enrichment for some particular form of RNA.
In plants, including cereals, total RNA is a mixture of many types of RNA and enrichment is generally required.
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Soilless nursery
Time of Update: 2020-11-18
For example 2 servings of grass charcoal, 1 serving of zircon mixed as a substation, seedling effect is better, seedling growth is strong, and the substation is more closely combined, not easy to disperse.
This nutrient block is beneficial for seed germination and seedling growth.
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Cheminformatic Analysis of High-Throughput Compound Screens
Time of Update: 2020-11-18
First, we introduce cheminformatic concepts for analyzing drug-like small molecule structures and their properties.
Finally, we discuss methods for interpreting screening hits by analyzing compound structures and induced phenotypes using similarity search and clustering approaches.
