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Assaying Effector Function in Planta Using Double-Barreled Particle Bombardment
Time of Update: 2020-11-18
The double-barreled gene gun device is an inexpensive and highly effective attachment that enables statistically significant data to be obtained with one-tenth the number of experimental replicates compared to conventional biolistic assays.
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Fragrant leaf geranium
Time of Update: 2020-11-18
The Soviet Union, Morocco and Algeria were also cultivated, but mainly pink leaf geraniums.
In the early 20th century, China introduced a small number of plants, as potted ornamental plants, after 1949 began to produce productive cultivation.
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The birch
Time of Update: 2020-11-18
in the dense forests of Shishan in guangxi, southeastern Yunnan and northern Vietnam, there is a very hard wood tree called birch.
Guangxi Longzhou County, a large birch, about 40 meters high, the trunk diameter of about 1 meter, age between 450-500 years old, this is probably the largest birch.
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Measurements of Viability Suitable for Plant Tissue Cultures
Time of Update: 2020-11-18
Viability is the capacity of a cell or organism to live, and the state of being viable is most commonly recognized by the growth of the studied tissues ( 1 - 3 ).
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Survey of Natural and Transgenic Gene Markers Used to Monitor Transposon Activity
Time of Update: 2020-11-18
Marker genes have played a critical role in the discovery of plant transposable elements, our understanding of transposon biology, and the utility of transposable elements as tools in functional genomics.
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Fruit Organ Cultures
Time of Update: 2020-11-18
Typically, however, when an isolated portion of the fruit tissue is introduced into a sterile environment, it immediately loses structural integrity and degenerates into a rapidly dividing callus mass ( 2 ).
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RNA Extraction from Cereal Vegetative Tissue
Time of Update: 2020-11-18
In some instances, total RNA is all that is required; however most applications will require the enrichment for some particular form of RNA.
In plants, including cereals, total RNA is a mixture of many types of RNA and enrichment is generally required.
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Cheminformatic Analysis of High-Throughput Compound Screens
Time of Update: 2020-11-18
First, we introduce cheminformatic concepts for analyzing drug-like small molecule structures and their properties.
Finally, we discuss methods for interpreting screening hits by analyzing compound structures and induced phenotypes using similarity search and clustering approaches.
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Assays for Effector-Mediated Suppression of Programmed Cell Death in Yeast
Time of Update: 2020-11-18
We use Bax and H 2 O 2 to induce cell death and mimic some PCD features that naturally appear during the development of multicellularSaccharomyces cerevisiae colonies and assay whether plant pathogen effectors can inhibit the process.
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Use of Microarray Analysis to Dissect the Plant Defense Response
Time of Update: 2020-11-18
Analysis of the large data sets produced from microarray experiments requires the application of statistical methods to define significant differences in gene expression, and computerized algorithms for pattern recognition.
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Entomopathogenic Nematodes
Time of Update: 2020-11-18
Entomopathogenic nematodes of the generaSteinernema and Hetero rhabditis (Nematoda: Rhabditida) have emerged as excellent insect biological control agents.
This discipline of insect pathology has made enormous strides since Glaser’s discovery more than 60 yr ago of nematodes infesting white grubs ( 1 ,2 ).
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Flower Organ Culture
Time of Update: 2020-11-18
Flower formation in tissue culture has been observed in several plant species, and arises from a variety of explant sources (2).
However, the factors responsible for flowering in culture have not been extensively studied (I).
Reasons for studying flower formation in tissue culture can be summarized as follows:
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Mycoherbicides
Time of Update: 2020-11-18
These epidemics all resulted from the accidental introduction of an exotic pathogen into areas where the host plant had been cultivated in the absence of the pathogen.
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Standardizing Seed Dormancy Research
Time of Update: 2020-11-18
Seeds are very attractive and convenient for molecular genetic studies that challenge principal biological phenomena related to the initiation and suppression of growth (e.g., germination and dormancy, respectively).
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Scent of rest (benzoin)
Time of Update: 2020-11-18
Growing more than 10 years of strong adult trees, in the summer, autumn two seasons of fat cutting.
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Transient Gene Expression in Protoplasts of Arabidopsis thaliana
Time of Update: 2020-11-18
The transfer of definedDNA segments mto plant cells 1s an established procedure to study regulation of gene expression ( 1 , 2 ).
Techmques for direct DNA transfer and transient expression analysis of introduced genes provide a convenient alternative to stable transformation procedures.
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Transformation of Barley (Hordeum vulgare L.) by Agrobacterium tumefaciens Infection of In Vitro Cultured Ovules
Time of Update: 2020-11-18
Agrobacterium -mediated transformation of in vitro cultured barley ovules is an attractive alternative to well-established barley transformation methods of immature embryos.
The ovule culture system can be used for transformation with and without selection and has successfully been used to transform cultivars other than Golden Promise indicating minor genotype dependency.
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Tobacco Transformation
Time of Update: 2020-11-18
For researchers interested in unraveling the roles of specific genes in particular pathways of growth and development, the introduction into plants of foreign genes and gene promoters linked to reporter genes allows the detailed study of the temporal, spatial, and quantitative expression of plant genes and the activities of associated regulatory sequences.
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Tung.
Time of Update: 2020-11-18
, there are six kinds of oil in the world, with the origin of China's three-year and millennium slugs are the most common.
Millennium learning famous wood oil, cause and peel wrinkles, so also known as turtle back, meaning long life hundred years old.
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Activation Tagging Using the Maize En-I Transposon System for the Identification of Abiotic Stress Resistance Genes in Arabidopsis
Time of Update: 2020-11-18
An activation tag (ATag) library of 800 lines was generated in Arabidopsis ecotype Columbia using theEn-I ( Spm ) transposon system.
The ATag lines were used in a forward genetics strategy to identify novel genes that confer resistance/tolerance to abiotic stresses.
