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Transient Gene Expression in Protoplasts of Arabidopsis thaliana
Time of Update: 2020-11-18
The transfer of definedDNA segments mto plant cells 1s an established procedure to study regulation of gene expression ( 1 , 2 ).
Techmques for direct DNA transfer and transient expression analysis of introduced genes provide a convenient alternative to stable transformation procedures.
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Entomopathogenic Nematodes
Time of Update: 2020-11-18
Entomopathogenic nematodes of the generaSteinernema and Hetero rhabditis (Nematoda: Rhabditida) have emerged as excellent insect biological control agents.
This discipline of insect pathology has made enormous strides since Glaser’s discovery more than 60 yr ago of nematodes infesting white grubs ( 1 ,2 ).
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Protein Damage and Repair Controlling Seed Vigor and Longevity
Time of Update: 2020-11-17
Analysis of the accumulation of isoaspartyl-containing proteins and its modulation by the PIMT repair pathway, using germination tests, immunodetection, enzymatic assays, and HPLC analysis, gives new insights in understanding controlling mechanisms of seed longevity and vigor.
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NPTII Assays for Measuring Gene Expression and Enzyme Activity in Transgenic Plants
Time of Update: 2020-11-17
The stable introduction of genes into plants through genetic engineering normally necessitates the use of a selectable marker, especially when the transformation frequency is low (e.g., 1.0 � 10 −3 to 10 −6 ).
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Strategies for Improving Vaccine Antigens Expression in Transgenic Plants: Fusion to Carrier Sequences
Time of Update: 2020-11-17
This chapter is devoted to the description of the methods utilized for the generation of transgenic plants expressing a canine parvovirus vaccine peptide or virus-like particles from a rabbit calicivirus.
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Peach-leaf coral Aucuba japonica
Time of Update: 2020-11-17
Small branches are thick and round.
The main point of : breeding with slugs, should be carried out during the plum rain.
Transplants should be carried out in spring or during the rainy season.
Its branches and leaves can be used for flowering.
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Immature Seeds and Embryos of Medicago truncatula Cultured In Vitro
Time of Update: 2020-11-17
Working with the model legumeMedicago truncatula , an in vitro protocol was developed for the culture of immature embryos that permits their development in a way comparable to that observed in plants.
In this chapter, the usefulness of this system for investigating embryo development in legumes is outlined.
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Rohansson
Time of Update: 2020-11-17
Planting, spring and autumn two seasons, spring selection dormant branches, autumn selection of semi-woody branches, 12-15 cm, inserted into the sand, soil half of the seedling bed, about 50-60 natural roots.
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Clonal Propagation of Softwoods
Time of Update: 2020-11-17
Plantlet production via organogenesis requires at least four stages: (1) bud induction on the explant, (2) shoot development and multiplication, (3) rooting of developed shoots, and (4) hardening of plantlets.
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DNA Purification from Multiple Sources in Plant Research with Homemade Silica Resins
Time of Update: 2020-11-17
Although different kits are available in the market allowing convenient DNA purification, the cumulative cost of purchasing multiple kits for a laboratory can be staggering.
Compared with the commercial kits, this protocol enables easy DNA purification from diverse sources with comparable yield and purity at negligible expenses.
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Canola
Time of Update: 2020-11-17
Canola and soybeans, peanuts and sesame seeds are the four major oil crops in China.
Canola is also one of the main honey crops.
canola is mainly distributed in the Yangtze River basin area, for two-year raw crops.
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Histochemical and Fluorometric Assays for uidA (GUS) Gene Detection
Time of Update: 2020-11-17
The aim of this chapter is to describe the basic protocols needed for GUS detection in a plant genetic transformation laboratory.
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Standardization of Data Processing and Statistical Analysis in Comparative Plant Proteomics Experiment
Time of Update: 2020-11-17
Despite the continuous technical advances and improvements in current 2-DE protocols, an adequate and correct experimental design and statistical analysis of the data tend to be ignored or not properly documented in current literature.
In this chapter, we describe a model procedure for a correct experimental design and a complete statistical analysis of proteomic dataset.
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Propagation, Storage, and Assays with Hyaloperonospora arabidopsidis: A Model Oomycete Pathogen of Arabidopsis
Time of Update: 2020-11-17
The oomycete pathogenHyaloperonospora arabidopsidis is a natural pathogen ofArabidopsis thaliana and a laboratory model for (1) understanding howArabidopsis responds to pathogen attack; (2) comparative and functional genomics of oomycetes; and (3) the molecular basis and evolution of obligate biotrophy.
arabidopsidis – Arabidopsis interaction.
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Use of Phospholipase A2 for the Production of Lysophospholipids
Time of Update: 2020-11-17
For this purpose, authentic LPL standards have to be synthesized from phosphoglycerides by PLA 2 digestion in vitro.
PLA 2 specifically hydrolyzes the fatty acid ester linkage in thesn -2-position of phospholipids to liberatesn -2-linked fatty acids and the corresponding LPL.
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Cryopreservation of Rice Tissue Cultures
Time of Update: 2020-11-17
For example, the production of transgenic plants of rice ( Oryza sativa L.) either by directDNA uptake into protoplasts or by particle bombardment is dependent on embryogenic callus or cell suspension cultures from which fertile plants can be regenerated ( 1 , 2 ).
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Analysis of Unfolded Protein Response in Arabidopsis
Time of Update: 2020-11-17
Monitoring the UPRin planta is an elemental approach to identifying regulatory components and to revealing molecular mechanisms of the plant UPR.
In this chapter, we provide protocols for the induction and analyses of plant UPR at a molecular level inArabidopsis .
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Medicago sativa
Time of Update: 2020-11-17
also known as alfalfa. Legume. Perennian herbs, about 1 high meters. Three out of the compound leaves, each other, small leaves inverted or inverted needle-shaped, the upper leaf edge has jagged;
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AFLP Fingerprinting of Arabidopsis
Time of Update: 2020-11-17
AFLP − (KeyGene, Wagenmgen, The Netherlands), is aDNA fingerprmtmg technique that visualizes DNA restriction fragments by polymerase chain reaction ( PCR ) amphfication ( 1 , 2 ).
The AFLP technique consists of three major steps:
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Pool fir
Time of Update: 2020-11-17
2 to 4 cm long; seed irregular triangle, slightly flat, red-brown, 1.3 to 1 .8cm, sharp ridge at the edge.
