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DNA Purification from Multiple Sources in Plant Research with Homemade Silica Resins
Time of Update: 2020-11-17
Although different kits are available in the market allowing convenient DNA purification, the cumulative cost of purchasing multiple kits for a laboratory can be staggering.
Compared with the commercial kits, this protocol enables easy DNA purification from diverse sources with comparable yield and purity at negligible expenses.
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NPTII Assays for Measuring Gene Expression and Enzyme Activity in Transgenic Plants
Time of Update: 2020-11-17
The stable introduction of genes into plants through genetic engineering normally necessitates the use of a selectable marker, especially when the transformation frequency is low (e.g., 1.0 � 10 −3 to 10 −6 ).
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Cryopreservation of Rice Tissue Cultures
Time of Update: 2020-11-17
For example, the production of transgenic plants of rice ( Oryza sativa L.) either by directDNA uptake into protoplasts or by particle bombardment is dependent on embryogenic callus or cell suspension cultures from which fertile plants can be regenerated ( 1 , 2 ).
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Tuber.
Time of Update: 2020-11-17
The end of the underground stem forms a puffy and irregular block shape, which is suitable for storing nutrients and wintering perverted stems.
The underground branches of the potato grow in the soil layer, and when elonged 9 to 12 cm, the ends are puffy, forming fat tubers with short sections.
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Utilizing Bimolecular Fluorescence Complementation (BiFC) to Assay ProteinProtein Interaction in Plants
Time of Update: 2020-11-17
BiFC is based on splitting the yellow fluorescent protein (YFP) into two nonoverlappingN -terminal (YN) and C-terminal (YC) fragments.
Each fragment is cloned in-frame with a gene of interest, enabling expression of a fusion protein.
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Pulsed-Field Gel Electrophoresis for Isolation of Full-Length Phytoplasma Chromosomes from Plants
Time of Update: 2020-11-17
Pulsed-field gel electrophoresis (PFGE) is a powerful technique for genomic studies of unculturable plant-pathogenic phytoplasmas, which enables separation of full-length phytoplasma chromosomes from contaminating host plant nucleic acids.
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In Vitro Induction of Infection-Related Hyphal Structures in Plant Pathogenic Fungi
Time of Update: 2020-11-17
In order for data on biological relevance to keep pace with accumulating genomic data, new biological assays need to be developed for several pathogenic fungi.
Accordingly, we have developed an in vitro assay that allows us to monitor morphological changes in hyphal development as the head blight pathogenFusarium graminearum infects wheat.
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Tepary Bean (Phaseolus acutifolius)
Time of Update: 2020-11-17
Tepary bean ( Phaseolus acutifolius L.
Selection of transgenic callus material is achieved through four to five passages on geneticin-containing medium, after which shoots are induced on medium without selection agent.
The protocol as described here has been applied to transform a cultivated variety of P.
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Hairy Lily Lilium dauricum
Time of Update: 2020-11-17
the main points of : the main planted bulbs, sub-ball 2 to 3 years flowering.
Planting large bulbs deep and removing buds can encourage the formation of more sub-balls.
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A Protocol for Transformation of Torenia
Time of Update: 2020-11-17
This chapter describes anAgrobacterium tumefaciens -mediated transformation protocol for torenia, a plant that has several useful characteristics and is primarily used for ornamental and experimental purposes.
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Sugar cane cultivation
Time of Update: 2020-11-17
planting period: sugar cane growth is mainly affected by temperature and variety, in Taiwan about 14 to 18 months, but the root can be shortened, only 12 months.
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Bacterial Assay to Study Plant Sensor Histidine Kinases
Time of Update: 2020-11-17
Cytokinins, like other phytohormones, act in plants as signaling molecules at very low concentrations.
coli strains revealed that a variety of cytokinin compounds may have signaling functions, but only with specific receptors.
This chapter describes the detailed protocol of a method employing transgenicE.
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Analysis of Geminivirus DNA Replication by 2-D Gel
Time of Update: 2020-11-17
The technique described was developed for the separation of begomovirusDNA .
DNA products resulting from and during geminiviral replication are characterized by the application of strand-specific separation and identification by strand-specific DNA probing of Southern blots.
The mapping of the initiation site of complementary-strand DNA synthesis, by this technique is also presented.
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Pigeonpea (Cajanus cajn (L.) Millsp.)
Time of Update: 2020-11-17
The development of plant transformation techniques has been a major breakthrough in overcoming constraints to achieve precision in genetic manipulation.
The development of efficient plant regeneration protocols is a prerequisite for recombinant technology to carry out genetic transformation.
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Directing Anthraquinone Accumulation via Manipulation of Morinda Suspension Cultures
Time of Update: 2020-11-17
Secondary metabolite accumulation in cell suspensions is the result of an interplay between primary metabolism, supplying the machinery, the energy, and the precursors, and the secondary metabolic pathways.
These interactions are best studied in cell suspensions that produce significant amounts of secondary metabolites.
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Quantification of Abscisic Acid, Cytokinin, and Auxin Content in Salt-Stressed Plant Tissues
Time of Update: 2020-11-17
Quantification of the hormone levels and determination of their ratios can reveal different plant strategies to cope with the stress, e.g., suppression of growth or mobilization of plant metabolism.
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Massive Indexed Parallel Identification of Transposon Flanking Sequences
Time of Update: 2020-11-17
The development of next-generation sequencing technologies has further facilitated the creation of flanking sequence collections derived from entire mutant populations.
Here we describe a highly efficient and widely applicable method that we developed to amplify, sequence, and identifydTph1 transposon flanking sequences from a library of 1000 Petunia W138 individuals simultaneously.
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Metabolite Profiling as a Functional Genomics Tool
Time of Update: 2020-11-17
Plants accumulate a very large number of small molecules (phytochemicals) with important functions in the ecology of plants and in the protection against biotic and abiotic stress conditions.
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Virus-Induced Gene Silencing Using Begomovirus Satellite Molecules
Time of Update: 2020-11-17
VIGS is induced by infecting a plant with a plant virus that has had its genome modified to include a sequence from the host gene to be silenced.
Here, we describe procedures for the use of these two gene-silencing vectors for VIGS in different hosts.
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Bimolecular Fluorescence Complementation (BiFC) to Study Protein-protein Interactions in Living Plant Cells
Time of Update: 2020-11-17
However, the study of protein complex formation in living plant cells has remained experimentally difficult and time-consuming and requires sophisticated technical equipment.
In this report, we describe a bimolecular fluorescence complementation (BiFC) technique for visualization of protein–protein interactions in plant cells.
