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Propagation, Storage, and Assays with Hyaloperonospora arabidopsidis: A Model Oomycete Pathogen of Arabidopsis

 Last Update: 2020-11-17
 Source: Internet
 Author: User

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The oomycete pathogen
Hyaloperonospora arabidopsidis
is a natural pathogen of
Arabidopsis thaliana
and a laboratory model for (1) understanding how
Arabidopsis
responds to pathogen attack; (2) comparative and functional genomics of oomycetes; and (3) the molecular basis and evolution of obligate biotrophy. Here, we describe procedures for propagation and long-term storage of
H. arabidopsidis
, which address complications arising from its biotrophic lifestyle that precludes growth on synthetic media. We also describe four assays that provide information on different facets of the
H. arabidopsidis
–
Arabidopsis
interaction.

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