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RNA Extraction from Cereal Vegetative Tissue

 Last Update: 2020-11-18
 Source: Internet
 Author: User

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protein extraction protocol

ribonucleic acid function

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Ribonucleic acid (RNA) extraction is the necessary first step in many protocols, primarily to investigate genes and gene expression. RNA comes in a variety of forms: total RNA, ribosomal RNA, messenger RNA (mRNA), and small interfering RNA (siRNA) to name a few. In some instances, total RNA is all that is required; however most applications will require the enrichment for some particular form of RNA. In plants, including cereals, total RNA is a mixture of many types of RNA and enrichment is generally required. In this protocol, the TRIzol
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method of RNA extraction from cereal leaf material is described, as it is a relatively simple technique.

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