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Cork Oak Trees (Quercus suber L.)
Time of Update: 2020-11-18
Embryos obtained from recurrent proliferating embryogenic masses are inoculated with AGL1 strain harbouring the plasmid pBINUbiGUSint, which carries thenpt II anduidA genes.
Evidence of stable transgene integration is obtained by polymerase chain reaction fornpt II anduidA genes, Southern blotting and expression of theuidA gene.
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Gene Characterization by Southern Analysis
Time of Update: 2020-11-18
The study of how genes are organized within the genome is a major step in the characterization of clonedDNA sequences and is important in the analysis of plant transformation.
The analysis may be complicated by the presence of between one and several hundred related sequences in the genome.
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Comparative Gel-Based Phosphoproteomics in Response to Signaling Molecules
Time of Update: 2020-11-18
Here, we describe how to take advantage of the availability of fluorescent dyes to stain for a selective class of proteins on the same gel for the detection of both phospho- and total proteomes.
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New Insights into the Control of Cell Growth
Time of Update: 2020-11-18
Undoubtedly, the function of the plant cell wall in the control of cell growth far exceeds its mechanical role.
With their biochemical nature yet unknown, the identification of molecular constituents of cell wall performance, and integrity control initially depends on a combination of genetic and physiological approaches.
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Phenotypic, Physiological, and Molecular Evaluation of Rice Chilling Stress Response at the Vegetative Stage
Time of Update: 2020-11-18
Recent physiological and molecular studies have shown that oxidative stress is the primary cause of early chilling injuries in rice and the differential responses of indica and japonica cultivars are defined to a large extent by gene expression related to oxidative signaling and defenses.
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Mutagenesis Techniques in Plant Tissue Cultures
Time of Update: 2020-11-18
Often these mutants have been obtained through selection procedures exploiting spontaneous mutations.
However, the use of mutagens in culture systems has been shown to result in higher frequencies of mutant cells.
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Pull-Down Analysis of Interactions Among Jasmonic Acid Core Signaling Proteins
Time of Update: 2020-11-18
Pull-down assays are key tools to test specific protein–protein interactions and have been particularly fruitful in jasmonate signaling research.
Pull-down assays contributed not only to shape this signaling pathway but also to identify the active jasmonate hormone.
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Regulation of the Mutator System of Transposons in Maize
Time of Update: 2020-11-18
TheMutator system has proved to be an invaluable tool for elucidating gene function via insertional mutagenesis.
Its high copy number, high transposition frequency, relative lack of insertion specificity, and ease of use has made it the preferred method for gene tagging in maize.
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Measurement of Abscisic Acid and Gibberellins by Gas Chromatography/Mass Spectrometry
Time of Update: 2020-11-18
Gas chromatography–mass spectrometry (GC-MS)-based analysis is an accurate and sensitive method to quantify plant hormones.
This method is commonly used for analysis of low-molecular-weight compounds, such as abscisic acid (ABA), gibberellins (GAs), auxins, and brassinosteroids.
Here, we describe a protocol for quantification of ABA and GAs.
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Callus Culture and Regeneration
Time of Update: 2020-11-18
Regeneration of plants by micropropagation of in vitro cultures can be achieved from organ primordia existing m shoot tips and axillary bud explants.
Alternatively, plants can be regenerated from unorganized callus tissues derived from different explants by dedifferentiation induced by exogenous growth regulators.
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High-Throughput Identification of Chemical Endomembrane Cycling Disruptors Utilizing Tobacco Pollen
Time of Update: 2020-11-18
Endomembrane cycling processes in plants remain mostly intractable through classical genetic interrogation.
Tobacco pollen, which is dependent upon endomembrane cycling for tube growth, provides a plant system that is amenable to high-throughput screening of chemical disruptors.
We describe here the process that allowed the identification of over 360 endomembrane cycling disruptors.
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Clivia spp.
Time of Update: 2020-11-18
Berry fuchsia, wide egg shape; Native to southern Africa.
Widely cultivated throughout China.
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Visualizing and Quantifying the In Vivo Structure and Dynamics of the Arabidopsis Cortical Cytoskeleton Using CLSM and VAEM
Time of Update: 2020-11-18
The cortical microtubules, and to some extent also the actin meshwork, play a central role in the shaping of plant cells.
With the aid of suitable computing techniques, quantitative information can be extracted from microscopic images and video sequences, providing insight into both architecture and dynamics of the cortical cytoskeleton.
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橙（Citrus sinensis）
Time of Update: 2020-11-18
Flowers of both sexes, white, single or several clusters are born in the leaf, 5 pieces of slivers, the base is connected, the male majority, the flower wire is synthesized array, born on the flower plate, the children's room multi-room.
Orange peel is medicinal again.
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Cyrtomium Fortunei
Time of Update: 2020-11-18
The root stem is short and upright, together with the leaf handle base is densely brown brown, hairy broad egg-shaped needle-shaped large scales, leaves oddly a feather-like; Leaf vein mesh, mesh has a small vein hidden inside.
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High-Throughput TILLING for Arabidopsis
Time of Update: 2020-11-18
In this chapter, we describe the basic methodology for high-throughput TILLING.
Gene segments are amplified using fluorescently tagged primers, and products are denatured and reannealed to form heteroduplexes between the mutated sequence and its wild-type counterpart.
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Use of Rolling-Circle Amplification for Large-Scale Yeast Two-Hybrid Analyses
Time of Update: 2020-11-18
Detection of protein–protein interactions on a large-scale has become a major focus of functional genomics after the completion of genome sequencing.
The information generated from these studies not only assembles proteins into signaling networks, but also reveals potential functions of uncharacterized proteins when their interacting partners have known functions.
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Bamboo.
Time of Update: 2020-11-18
Perennation, woody underground stems, straw wood, there are obvious sections, between the festival often hollow.
Straw can be used for a variety of construction materials, bamboo can be woven a variety of utensils.
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Karyotyping Methods for Fungi
Time of Update: 2020-11-18
The germ tube burst method is a technique to separate fungal chromosomes of any size for chromosome number determination as well as in situ hybridization.
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Protoplast Isolation, Culture, and Plant Regeneration from Passiflora
Time of Update: 2020-11-18
Interspecific sexual hybridization has been attempted inPassiflora breeding programs, using wild-type germplasms to transfer disease resistance and other potentially desirable traits into cultivated species.
Indeed, novel fertile somatic hybrids have been produced betweenP.
Somatic hybrid plants have also been produced betweenP edulis fv.
