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Peony (Paeonia Suffruticosa)
Time of Update: 2020-11-18
Famous ornamental plants, one of the top ten flowers in China, the cultivation 2000.
Luoyang and many other cities have been selected as city flowers.
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Solution Structure Probing of RNA Structures
Time of Update: 2020-11-18
Computer modeling based on free energy minimizing principles is generally used as an initial approach to predict potential RNA secondary structures in a sequence.
This process involves treating the RNA of interest with enzymes or chemicals that modify RNA differentially based on its secondary structure.
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The CRE/lox System as a Tool for Developmental Studies at the Cell and Tissue Level
Time of Update: 2020-11-18
Implementation of several site-specific recombination systems such as CRE/ lox has created powerful tools to study the role of many genes at the cellular level.
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Fluorescence-Activated Cell Sorting in Plant Developmental Biology
Time of Update: 2020-11-18
Understanding the development of an organ requires knowledge of gene, protein, and metabolite expression in the specific cell types and tissues that comprise the organ.
This is a powerful method to examine biological functions of specific cell types and tissues with a systems biology approach.
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Use of Cosmid Libraries in Plant Transformations
Time of Update: 2020-11-18
The high efficiency of in vitro lambda packaging allows a cosmid library with large inserts to be more easily constructed than a simple plasmid library, which relies on transformation of competentEscherichia coli cells.
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In Vitro Culture and Germination of Terrestrial Asian Orchid Seeds
Time of Update: 2020-11-18
In this chapter, we document the timing of seed collection and pretreatments for improving in vitro germination of some terrestrial Asian orchids.
The process of in vitro germination is demonstrated, including (1) the culture of immature seeds; (2) the culture of mature seeds; and (3) subsequent seedling development.
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Biolistic-Mediated Production of Transgenic Oil Palm
Time of Update: 2020-11-18
The effectiveness of mannose (using phosphomannose isomerase [ pmi ] gene) as a positive selection agent to preferably allow the growth of transformed oil palm embryogenic calli was successfully evaluated.
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Isolation of Whole Cell (Total) RNA
Time of Update: 2020-11-18
Efficient extraction of high quality RNA from a variety of plant tissues is an important first step in many procedures, such as analysis of gene expression, c DNA library construction, and in vitro translation.
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Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays
Time of Update: 2020-11-18
In addition to the isolation of thylakoids, this chapter also describes the experimental steps of both protein translocation across the thylakoid membrane and protein integration into the membrane.
Protein translocation and integration can be analysed by the radioactive labelling of substrate proteins using an in vitro transcription and translation system.
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Cucumovirus Isolation and RNA Extraction
Time of Update: 2020-11-18
The genus includes cucumber mosaic virus (CMV, the type species), peanut stunt virus (PSV), and tomato aspermy virus (TAV) (reviewed inrefs.
Symptoms for all of the cucumoviruses may include stunting, mosaic, and leaf distortion, and may range from mild to severe.
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Anhua Yuntaishan
Time of Update: 2020-11-18
Yuntaishan, originally from Anhua County, Hunan Province, is now distributed in Xiang zhong, Xiangdong, Xiangbei and other major tea areas.
Some of these large leaf types, to the origin of Yuntaishan in the majority, so there are also Yuntaishan large leaf species.
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Stable Transformation of Plants
Time of Update: 2020-11-18
This chapter provides an overview of the main steps in the process to produce stably transformed plants.
We focus here on DNA delivery by particle bombardment asAgrobacterium -mediated transformation methods for wheat are reported elsewhere ( 29 ).
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Microarrays for Universal Detection and Identification of Phytoplasmas
Time of Update: 2020-11-18
Detection and identification of phytoplasmas is a laborious process often involving nestedPCR followed by restriction enzyme analysis and fine-resolution gel electrophoresis.
The present protocol describes a microarray-based method for identification of phytoplasmas to 16Sr group level.
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Flax.
Time of Update: 2020-11-18
Flowering to maturity with less rain and sufficient lighting is beneficial to the nutritional growth and fiber development of hemp stems.
Two coarse hemp can be used as carpet and high-quality paper raw materials.
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ISSR: A Reliable and Cost-Effective Technique for Detection of DNA Polymorphism
Time of Update: 2020-11-18
These marker systems differ in their advantages and disadvantages, so it is imperative to keep in mind all the pros and cons of the technique while selecting one for the problem to be addressed.
We have outlined here the whole methodology of this technique with an example ofTribulus terrestris as case study.
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Lily.
Time of Update: 2020-11-18
lily is available for medicinal use in the Chinese Han Dynasty's Book of Shen Nong Herbs.
From the beginning of the 17th century to the 18th century, lily of American and Chinese origin was introduced to Europe.
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Agrobacterium-Mediated Transformation of Potato Genotypes
Time of Update: 2020-11-18
The potato is ideally suited for improvement by modern genetic manipulation methods, as it is highly amenable to a wide range of various tissue-culture techniques ( 1 ).
Genetic transformation, however, provides a more direct and controlled method for manipulating the genome of potato for both basic and applied research purposes ( 3 ).
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Appendix A: The Components of the Culture Media
Time of Update: 2020-11-18
The success in the technology and application of plant tissue culture is greatly influenced by the nature of the culture medium used.
A better understanding of the nutritional requirements of cultured cells and tissues can help to choose the most appropriate culture medium for the explant used.
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Prajna.
Time of Update: 2020-11-18
Sowing, often in may-June indoor pot planting after 5-6 days germination, live seedlings 8-10 years can flower.
Sow small seedlings as a spike, graft seedlings 3-4 years can flower.
There is a dormant period in winter, when the pot soil should be kept slightly dry.
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Cork Oak Trees (Quercus suber L.)
Time of Update: 2020-11-18
Embryos obtained from recurrent proliferating embryogenic masses are inoculated with AGL1 strain harbouring the plasmid pBINUbiGUSint, which carries thenpt II anduidA genes.
Evidence of stable transgene integration is obtained by polymerase chain reaction fornpt II anduidA genes, Southern blotting and expression of theuidA gene.
