-
Java amomum
Time of Update: 2020-11-19
half-shaded plants, like high temperature and humid low-altitude forest environment.
It is advisable to plant bananas and other shade trees, to wait for the white bean seedlings about 30cm high, divided into 3-4 plants, you can be planted out of the nursery.
-
A Straightforward Protocol for Electro-transformation of Phytophthora capsici Zoospores
Time of Update: 2020-11-19
Here, we describe a straightforward protocol that allows rapid transformation ofPhytophthora capsici , an emerging model in oomycete biology.
This protocol relies on electroporation-assisted uptake ofDNA in to motile zoospores and allows the rapid identification and characterization of genetically stable transformants.
-
Fluorescence Cross-Correlation Spectroscopy of Plant Proteins
Time of Update: 2020-11-19
Because it simply makes use of the coincidental movement of two molecules, it could avoid the complexity that sometimes occurs in other fluorescent techniques such as fluorescence resonance energy transfer.
Determination in plant cells is also mentioned briefly.
-
Using the Yeast Two-Hybrid System to Identify ProteinProtein Interactions
Time of Update: 2020-11-19
The rationale of the yeast two-hybrid system relies on the physical separation of theDNA -binding domain from the transcriptional activation domain of several transcription factors.
When a protein encoded by the cDNA library binds to the bait, both activities of the transcription factor are rejoined resulting in transcription from a reporter gene.
-
Appendix B: Plant Biotechnology and Tissue Culture Resources in the Internet
Time of Update: 2020-11-19
This appendix compiles a list of useful Internet sites for cell culture scientists.
We anticipate that some of these sites will be included among the reader’s favorites (if they are not already).
-
Back to Osborne. Sequential Protein Extraction and LC-MS Analysis for the Characterization of the Holm Oak Seed Proteome
Time of Update: 2020-11-19
Thus, according to our results, and even considering that they depend on the experimental system carried out (plant, yeast, fungi, or bacteria), the best protein extraction protocol yielded less than 20 % of the total amount of proteins, as determined by the Kjeldahl method.
-
Money loose
Time of Update: 2020-11-19
Leaf strips, which grow each other on long branches, are formed in 15-30 wheeled clusters on short branches, and the leaves are 2-5.5cm long.
Transplantation or planting of trees should be carried out before germination, otherwise it is not easy to survive.
-
In Vitro Propagation of Ornamental Myrtus (Myrtus communis)
Time of Update: 2020-11-19
In this species, there is a great variability in the natural germplasm around the Mediterranean coasts for type and size of fruit, plant architecture, leaf size and internode length.
IAA or IBA at 0.5 mg/L increased the rooting percentage and noticed differences in root number and length.
-
Methods for Functional Proteomic Analyses
Time of Update: 2020-11-19
In this chapter we describe a typical proteomics experiment, using examples from our laboratory: the separation of complex mixtures of proteins by 2-dimensional electrophoresis and subsequent identification of a protein spot by mass spectrometry with two commonly used instruments: MALDI-QqTOF and ESI-ion trap.
-
Transient Expression of Antibodies in Plants Using Syringe Agroinfiltration
Time of Update: 2020-11-19
While vacuum-based agroinfiltration has been brought to large scale to meet the cost, speed and surge capacity requirements for vaccine and therapeutic production, the more accessible and affordable syringe agroinfiltration procedure still represents a fast and high-yielding approach to recombinant protein production at lab scale.
-
Analyzing Meiotic Chromosomes in Rice
Time of Update: 2020-11-19
Visualization of meiotic chromosomes has now become routine in cytogenetic studies in this species.
This chapter provides protocols on basic meiotic chromosome preparation, FISH analysis, and immunocytology in rice.
-
Microscopic Analysis of Arabidopsis Ovules
Time of Update: 2020-11-19
Ovules are the major female reproductive organs in higher plants.
Furthermore, ovules ofArabidopsis thaliana are successfully used as model system to study plant organogenesis.
Here we describe two microscopic techniques to analyze ovule development in Arabidopsis.
-
Arabidopsis thaliana Floral Dip Transformation Method
Time of Update: 2020-11-18
The availability of the method has had a transformative effect on the overall practice of plant molecular biology, as the generation and analysis of large numbers of transgenic plants is now routine in hundreds of laboratories.
-
Binary Vectors and Super-binary Vectors
Time of Update: 2020-11-18
Whereas vectors with compatible selectable markers and convenient cloning sites are usually the top criteria when inserting gene fragments shorter than 15 kb, the capability of maintaining a large DNA piece is more important for consideration when introducing DNA fragments larger than 15 kb.
-
Dragon boat flowers
Time of Update: 2020-11-18
Hi high temperature wet and sunny environment, cold, semi-yin, requiring rich in humus, loose, fertile acidic soil, winter temperature is not less than 0 degrees Celsius.
Sowing, winter seeding, spring planting the following year, 20-25 days after seeding germination, 3-4 pairs of leaves can be transplanted.
-
Hericium erinaceus.
Time of Update: 2020-11-18
Born on birch, walnuts and other standing and rotting wood; Monkey head bacteria contain polysaccharides and amino acids , for the common edible bacteria, the drug is beneficial to five dirty, aid digestion, tonic and anti-cancer effect.
-
Analyzing the In Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters
Time of Update: 2020-11-18
Exogenous application of biologically important molecules for plant growth promotion and/or regulation is very common both in plant research and horticulture.
Consequently, it is often very useful to know the in vivo status of applied compounds to connect them with some of the regulatory events in plant developmental processes.
-
Cotton.
Time of Update: 2020-11-18
Flowers, will automatically change color, the beginning of white, and then yellow, afternoon red, the next day purple red, thank gray-brown.
After gin processing, called cotton, used for spinning fabrics.
cotton is generally white, after dyeing to weave colorful flower cloth.
-
A One-Step Affinity-Purification Protocol to Purify NB-LRR Immune Receptors from Plants That Mediate Resistance to Fungal Pathogens
Time of Update: 2020-11-18
Nucleotide-binding, leucine-rich repeat (NB-LRR) immune receptors from plants confer resistance to fungal pathogens and many other pathogenic organisms.
Here we describe a protocol to affinity-purify recombinant NB-LRR immune receptors, fused to the streptavidin-binding peptide tag.
-
The CRE/lox System as a Tool for Developmental Studies at the Cell and Tissue Level
Time of Update: 2020-11-18
Implementation of several site-specific recombination systems such as CRE/ lox has created powerful tools to study the role of many genes at the cellular level.
