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An Affinity Pull-Down Approach to Identify the Plant Cyclic Nucleotide Interactome
Time of Update: 2020-11-19
In plants however, few CNBPs have been identified as homology searches fail to return plant sequences with significant matches to known CNBPs. Recently, affinity pull-down techniques have been successfully used to identify CNBPs in animals and have provided new insights into CN signaling.
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Tianmen winter
Time of Update: 2020-11-19
Breeding and cultivation: days of winter breeding to seed and sub-plant-based, sowing can pick ripe oar fruit after dry storage, to the spring of the next year sowing in shallow basins, covered with glass or plastic film, to maintain the temperature of 20 degrees C-25 degrees C and pelvic soil moist, 30 days or so can germinate, to be seeded 5 cm - 10 cm when the basin.
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Bamboo tea
Time of Update: 2020-11-19
bamboo barrel tea for the cylinder shape, the shape of the column smooth, green and green, after brewing both tea aroma, and bamboo fragrance, cool thirst.
After cooling, cut open the bamboo barrel, with the specifications of the trademark of the devert paper packaging.
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Reaping the Benefits of SAGE
Time of Update: 2020-11-19
This chapter introduces the methods and software tools that are available for researchers to analyze gene expression through SAGE analysis.
A detailed examination of SAGE analysis inArabidopsis thaliana using the publicly available analysis tool, SaskSAGE, is provided.
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Liquid Chromatography Tandem Mass Spectrometry for Measuring 13C-labeling in Intermediates of the Glycolysis and Pentose Phosphate Pathway
Time of Update: 2020-11-19
This chapter describes a procedure to analyze13 C-labeled phosphorylated compounds by liquid chromatography tandem mass spectrometry. Phosphorylated compounds, intermediaries of the glycolysis and p
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Assaying Levels of Virus with Local Lesion Hosts
Time of Update: 2020-11-19
A number of methods have been used for the quantitation of virus within infected plants.
These methods are all based on a physical aspect of the virus particle, and all measure total amounts of virus (or viral component), regardless of infectivity.
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Cloning of DNA Fragments: Ligation Reactions in Agarose Gel
Time of Update: 2020-11-19
Ligation reactions to ligate a desiredDNA fragment into a vector can be challenging to beginners and especially if the amount of the insert is limiting.
The use of rapid ligation buffer and incubation of the ligation reaction at room temperature greatly increase the efficiency of the ligation reaction even for blunt-ended ligation.
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Selection of Antimetabolite Resistant Mutants
Time of Update: 2020-11-19
Mutants resistant to chemicals that inhibit growth (antimetabolites) are the most readily selected in plant cell cultures.
Progress in mutant selection has been reviewed extensively in recent years ( 1 - 4 ).
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Emerging Mass Spectrometry-Based Technologies for Analyses of Chromatin Changes: Analysis of Histones and Histone Modifications
Time of Update: 2020-11-19
The use of unique fragmentation techniques in FTICR-MS provides a viable option for the study of labile modifications.
In this chapter, we provide a detailed description of the analytical tools – mass spectrometry in particular – that are used to characterize modifications on peptides and proteins.
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Gene and Enhancer Traps for Gene Discovery
Time of Update: 2020-11-19
With this system, genes can be identified based solely on the expression pattern of an inserted reporter gene.
The use of a reporter gene, such as β-glucuoronidase ( GUS ), provides a very sensitive assay for the identification of tissue- and cell-type specific expression patterns.
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Analysis of Fatty Acid Amide Hydrolase Activity in Plants
Time of Update: 2020-11-19
N -Acylethanolamines (NAEs) are fatty acid derivatives amide-linked to ethanolamine.
NAEs vary in chain lengths and numbers of double bonds and generally reflect the fatty acids found in membrane lipids in the tissues in which they reside.
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Use of Hairpin RNA Constructs for Engineering Plant Virus Resistance
Time of Update: 2020-11-19
Genetically engineered resistance to protect plants against virus infections can be based on protein- and RNA-mediated defense mechanisms.
The most efficient means to produce such dsRNA in transgenic plants is the introduction and expression of hairpin (hp) RNA constructs.
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cDNA Library Construction for the Lambda ZAP-Based Vectors
Time of Update: 2020-11-19
Because the vast majority of plant viruses have a positive-sense RNA genome, which acts as the viral mRNA, the RNA must first be converted into c DNA before cloning, amplification, and subsequent manipulation.
Secondary structure of mRNA molecules can cause the synthesis of truncated cDNA fragments.
Other potential difficulties include DNA molecules contaminating the mRNA sample.
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Encapsulation of In Vitro-Derived Explants: An Innovative Tool for Nurseries
Time of Update: 2020-11-19
This technology represents a further and promising tool for exchange of plant material between private and public plant tissue culture laboratories, for short- and medium-term storage of valuable plant material and for use of in vitro-derived or micropropagated propagules directly in farm or in nurseries.
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Sterile Techniques in Arabidopsis
Time of Update: 2020-11-19
First, large numbers of mutagenized seed can be easily scored for vartability, and secondly, plants in Petri plates can be grown constantly under completely defined condmons.
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2DE Analysis of Forest Tree Proteins Using Fluorescent Labels and Multiplexing
Time of Update: 2020-11-19
Nowadays, two-dimensional electrophoresis remains a powerful tool to explore the plant proteome and to unravel changes in protein abundance between samples.
This technique may be applied to a wide array of plant stress studies, among others to trees.
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Spinal cord.
Time of Update: 2020-11-19
Some plant stems mature earlier, while others continue to stretch, causing the myelin to rupture, forming cavities such as the hollow stems of the undergraduate, umbrella, gourd, etc.
Some plants can also be seen during the festival with a series of flaky myelin tissues (e.g. walnut genus, maple genus).
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Sequential Replicas for In Vivo Imaging of Growing Organ Surfaces
Time of Update: 2020-11-19
Sequential replica method facilitates in vivo imaging of plant surface and provides data sufficient for detailed computation of geometry and growth.
Images obtained from casts can be further used for data extraction, comprising 3D reconstruction and computation of local geometry and cell growth parameters.
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Extraction, Separation, and Analysis of Plant Phosphoinositides and Complex Glycolipids
Time of Update: 2020-11-19
Progress in the understanding of how cells perceive signals and how the perception of specific signals leads to cellular responses has been dramatic over the last decade.
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Dehydration Tolerance in Plants
Time of Update: 2020-11-19
Dehydration tolerance in plants is an important but understudied component of the complex phenotype of drought tolerance.
In this chapter we define what dehydration tolerance is and how it can be assessed, important prerequisites to understanding the response of a plant to water loss.
