-
Micropropagation of Banana
Time of Update: 2020-11-19
AAA) is propagated vegetatively and can be rapidly and efficiently propagated by micropropagation.
Conventional micropropagation techniques, however, may be too costly for commercial purposes.
Our laboratory has found that depending on the combination of culture vessel and gelling agent more economic methods can be chosen for successfully micropropagating banana.
-
In Vitro Propagation of Fraser Photinia Using Azospirillum-Mediated Root Development
Time of Update: 2020-11-19
However, photinia micropropagation is an effective alternative to traditional in vivo propagation which is affected by the seasonal supply of cuttings, the long time required to obtain new plants, and the difficulties in rooting some clones.
-
tonsil species resources (germplasm resources of almond)
Time of Update: 2020-11-19
Most of the cultivated species currently used in the main countries of tonsils are hybrids.
A small amount of tonsil cultivation in Xinjiang, China, includes dozens of farm varieties and types.
Several wild species of tonsils in China are shrubs.
-
The Use of Recombinant Inbred Lines (RILs) for Genetic Mapping
Time of Update: 2020-11-19
This is the case when cloned sequences have to be mapped.
The mapping of a specific DNA polymorphism is more effictent when one can use populations that have already been scored for many markers.
-
Electroporation of Brassica
Time of Update: 2020-11-19
A main objective of electroporation is to transform cells by stable incorporation and expression of foreign genetic material.
Incorporation may result in transformation with marker genes, as well as desired traits from a breeder’s point of view (e.g., male sterility, virus resistance, insect resistance).
-
The Detection of Neomycin Phosphotransferase Activity in Plant Extracts
Time of Update: 2020-11-19
Therefore, assays specific for neomycin phosphotransferase require a protein separation prior to the reaction and/or a separation of the reaction products.
Immunopurification of the NPTII enzyme ( 10 ) makes the assay sensitive and specific, but requires some additional handling and expensive chemicals.
-
Functional Analysis of Transcription Factors by Microparticle Bombardment
Time of Update: 2020-11-19
The second plasmid includes a reporter luciferase (LUC) gene transcribed from a promoter containing the TF binding site(s).
The third plasmid contains a reference β-glucuronidase (GUS) gene transcribed from the 35S-CaMV promoter, which is used to normalize data derived from independent experiments.
-
Measuring In Vitro Extensibility of Growing Plant Cell Walls
Time of Update: 2020-11-19
This article summarizes the theory and practical aspects of measuring cell wall properties by four different extensometer techniques and how the results of these methods relate to the concept and ideal measurement of cell wall extensibility in the context of cell growth.
-
Citronella.
Time of Update: 2020-11-19
plant height 60 to 150 cm, strong division, thick, the base has residual leaves; As an economic cultivation of the champs are mainly two kinds: Javanese-type fennel leaves long and wide, high yield, oil yield of about 1.3%, aldehyde, alcohol content is high, in the world's champs production has an important position.
-
Magnolia
Time of Update: 2020-11-19
Sub-plants, spring and autumn can be carried out, dig out the thick branches of the mother plant, and prune the root system and short branches.
Cultivation: can be carried out in autumn or early spring before flowering, seedlings with mud stains, seedlings must bring soil balls.
-
Little flower tequila
Time of Update: 2020-11-19
However, the small flower tequila germination force is poor, the growth of fewer children, so the breeding coefficient of the sub-plant is very low.
cultivation: Small flower tequila seedlings grow slowly, long-term do not need adequate watering, pot soil to maintain a little dry as well.
-
Detection of Double-Stranded RNA Elements in the Plant Pathogenic Fungus Rhizoctonia solani
Time of Update: 2020-11-19
Many species of fungi have been shown to harbor double-stranded RNA (dsRNA) elements.
A single fungal isolate ofRhizoctonia solani may have as many as five different dsRNA elements within them.
The presence of specific dsRNA elements influence pathogenicity in host plants.
-
Expression Analysis of Jasmonate-Responsive Lectins in Plants
Time of Update: 2020-11-19
TheNicotiana tabacum lectin, also designated Nictaba, is a nucleocytoplasmic carbohydrate-binding protein produced in tobacco leaves after application of specific jasmonates and upon insect herbivory.
Here, we describe different techniques by which lectin production can be induced through exogenous jasmonate application on tobacco plants.
-
Custom Knock-Outs with Hairpin RNA-Mediated Gene Silencing
Time of Update: 2020-11-19
Hairpin (hpRNA)-mediated gene silencing exploits a cellular mechanism that recognizes double-stranded RNA (dsRNA) and subjects it and its corresponding mRNA to a sequence-specific degradation.
Vectors, such as pHELLSGATE, that are based on the Gateway� technology are suitable for high-throughput gene silencing.
-
Methods for Engineering Resistance to Plant Viruses
Time of Update: 2020-11-19
For plant RNA viruses, virus complementaryDNA sequences shown to confer resistance include wild-type genes, mutated genes that produced truncated protein products, and nontranslatable sense or antisense transcripts to various regions of the virus genome.
-
Probing Circadian Rhythms in Chlamydomonas rheinhardtii by Functional Proteomics
Time of Update: 2020-11-19
These cells were then used for isolating basic proteins by heparin-affinity chromatography, separating them on two-dimensional PAGE and comparing the amount of their expression at four different time-points of subjective day and night.
-
Tianmen winter
Time of Update: 2020-11-19
Breeding and cultivation: days of winter breeding to seed and sub-plant-based, sowing can pick ripe oar fruit after dry storage, to the spring of the next year sowing in shallow basins, covered with glass or plastic film, to maintain the temperature of 20 degrees C-25 degrees C and pelvic soil moist, 30 days or so can germinate, to be seeded 5 cm - 10 cm when the basin.
-
Expression of Artificial MicroRNAs in Physcomitrella patens
Time of Update: 2020-11-19
During the last decade, the mossPhyscomitrella patens emerged as a model plant for functional gene analysis based on its unique ability to integrateDNA into the nuclear genome by homologous recombination which allows for the generation targeted gene knockout mutants.
-
Light wood
Time of Update: 2020-11-19
A 10-year-old light wood can be up to 16 meters high and 50 to 60 centimeters in diameter.
is one of the world's fastest growing tree species, a year can grow to five or six meters, diameter of 5-13 cm.
-
Organophosphorus Hydrolase: A Multifaceted Plant Genetic Marker Which Is Selectable, Scorable, and Quantifiable in Whole Seed
Time of Update: 2020-11-19
Organophosphorus hydrolase (OPH, EC 3.1.8.1) provides a novel function as an alternative genetic marker system for use in many types of plant transformations.
