Probing Circadian Rhythms in Chlamydomonas rheinhardtii by Functional Proteomics
-
Last Update: 2020-11-19
-
Source: Internet
-
Author: User
Search more information of high quality chemicals, good prices and reliable suppliers, visit
www.echemi.com
In the unicellular flagellated green alga
Chlamydomonas reinhardtii
several processes are regulated by the circadian clock. To study circadian controlled processes, the cell’s clock is synchronized in a 12 h light–12 h dark cycle (LD12:12) before the cells are released into constant conditions of dim light and temperature. Under these free-running conditions circadian rhythms will continue with a period of about 24 h and cells can be harvested during specific time-points of subjective day and night. These cells were then used for isolating basic proteins by heparin-affinity chromatography, separating them on two-dimensional PAGE and comparing the amount of their expression at four different time-points of subjective day and night. Among 230 proteins, we could find two proteins whose expression level changed more than fourfold throughout the circadian cycle. These proteins were identified as a protein disulfide isomerase (PDI)-like protein and a tetratricopeptide repeat (TPR) protein by liquid-chromatography-electrospray ionization mass spectrometry (LC-ESI-MS).
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to
service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.
