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Microarrays for Universal Detection and Identification of Phytoplasmas

 Last Update: 2020-11-18
 Source: Internet
 Author: User

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ecori restriction enzyme

agarose gel electrophoresis

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Detection and identification of phytoplasmas is a laborious process often involving nested
PCR
followed by restriction enzyme analysis and fine-resolution gel electrophoresis. To improve throughput, other methods are needed. Microarray technology offers a generic assay that can potentially detect and differentiate all types of phytoplasmas in one assay. The present protocol describes a microarray-based method for identification of phytoplasmas to 16Sr group level.

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