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Regulation of the Mutator System of Transposons in Maize

 Last Update: 2020-11-18
 Source: Internet
 Author: User

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plasmid copy number

sanger sequencing method

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The
Mutator
system has proved to be an invaluable tool for elucidating gene function via insertional mutagenesis. Its high copy number, high transposition frequency, relative lack of insertion specificity, and ease of use has made it the preferred method for gene tagging in maize. Recent advances in high throughput sequencing of insertion sites, combined with the availability of large numbers of pre-mutagenized and sequence-indexed stocks, ensure that this resource will only be more useful in the years ahead.
Muk
is a locus that can silence
Mu
-active lines, making it possible to ameliorate the phenotypic effects of high numbers of active
Mu
transposons and reduce the copy number of these elements during introgressions.

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