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    Home > Biochemistry News > Plant Extracts News > Protoplast Isolation, Culture, and Plant Regeneration from Passiflora

    Protoplast Isolation, Culture, and Plant Regeneration from Passiflora

    • Last Update: 2020-11-18
    • Source: Internet
    • Author: User
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    The family
    Passifloraceae
    contains over 580 woody or herbaceous species (
    1
    ), the majority of species within the genus
    Passiflora
    being found in tropical South America.
    Passiflora eduhs
    fv. flavicarpa is considered to be the most important species (
    2
    ) because of its value in the fruit juice industry. Additionally, this species is resistant to the soil-borne pathogen
    Fusarium oxysporum
    and, consequently, is frequently used as a rootstock onto which is grafted
    P edulis
    Sims. Interspecific sexual hybridization has been attempted in
    Passiflora
    breeding programs, using wild-type germplasms to transfer disease resistance and other potentially desirable traits into cultivated species. However, fertile hybrids have been difficult to obtain (
    3
    ). Somatic hybridization provides a means of circumventing such sexual incompatibilities. Indeed, novel fertile somatic hybrids have been produced between
    P. eduhs
    fv. flavicarpa and
    Passiflora incarnata
    (
    4
    ). Since the latter species can survive winter temperatures of −16�C, such transfer of cold tolerance to the commercial crop would permit cultivation in more temperate climates (
    5
    ). Somatic hybrid plants have also been produced between
    P edulis
    fv. flavicarpa and
    Passiflora amethystina, Passiflora cincinnata, Passiflora giberti
    , and
    Passiflora alata
    , respectively (
    6
    ).
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