 Home > Biochemistry News > Plant Extracts News > High-Throughput TILLING for Arabidopsis

High-Throughput TILLING for Arabidopsis

 Last Update: 2020-11-18
 Source: Internet
 Author: User

Tags

high temp bearings

acetaminophen high

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Targeting induced local lesions in genomes (TILLING) is a general strategy for identifying induced point mutations that can be applied to almost any organism. In this chapter, we describe the basic methodology for high-throughput TILLING. Gene segments are amplified using fluorescently tagged primers, and products are denatured and reannealed to form heteroduplexes between the mutated sequence and its wild-type counterpart. These heteroduplexes are substrates for cleavage by the endonuclease CEL I. Following cleavage, products are analyzed on denaturing polyacrylamide gels using the LI-COR
DNA
analyzer system. High-throughput TILLING has been adopted by the
Arabidopsis
TILLING Project (ATP) to provide allelic series of point mutations for the general
Arabidopsis
community.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

Beta-Thujaplicin499-44-5

CAS No.: 499-44-5

API Grade

$100/KG EXW

Irinotecan hydrochloride CAS 100286-90-6

CAS No.: 100286-90-6

Pharmaceutical Grade

$100/KG EXW

Stachydrine hydrochloride CAS 4136-37-2

CAS No.: 4136-37-2

Pharmaceutical Grade

$100/KG EXW

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

single use technology(195)

announcements today(81)

ups united states(221)

united states logo(227)

80 20 company(94)

healthy food name(42)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.