-
Bean potatoes
Time of Update: 2020-11-21
Bean potato root has two types of flat circle and spindle shape, the former is small, delicate texture, early ripe, produced in Guizhou, Sichuan, Yunnan and other places; the yield and quality of soybean potato is determined to a large extent by the nature of the soil, with a higher degree of weathering, good breathability, good drainage of yellow soil.
-
Electrophoretic Mobility Shift Assay for the Analysis of Interactions of Jasmonic Acid-Responsive Transcription Factors with DNA
Time of Update: 2020-11-21
The assay can be used to test DNA binding of either purified or recombinant proteins or uncharacterized binding activities present in crude protein extracts from plant cells or nuclei.
It allows the determination of the abundance, affinity, association rate constants, dissociation rate constants, and binding specificity of DNA-binding proteins.
-
Liquid In Vitro Culture for the Propagation of Arundo donax
Time of Update: 2020-11-21
The buds, attached to the stems are cultured in stationary liquid MS culture medium, supplemented with indole 3-acetic acid and kinetin.
Contrary to what happens in semisolid medium where roots are not formed, the plants cultured in liquid medium are whole plants with shoots and roots that develop at the same time.
-
Localization of Viral Proteins in Plant Cells: Protein Tagging
Time of Update: 2020-11-21
Methods are provided for (i) producing the constructs for transient expression from plasmids or virus-based vectors, (ii) introduction of constructs to plant epidermal cells; (iii) imaging of the expressed proteins by CLSM and image processing, and (iv) studying the expression in the presence of agents that affect the integrity or function of cytoskeletal elements.
-
In Vitro Propagation of Succulent Plants
Time of Update: 2020-11-21
Tissue-culture techniques have been applied to a wide range of succulents, and the relevant literature has recently been reviewed ( 1 , 2 ).
Here we describe methods for the micropropagation of cacti and other succulents that have been developed at Kew for overcoming the problems mentioned above.
-
Identification and Characterization of Quantitative Trait Loci that Control seed Dormancy in Arabidopsis
Time of Update: 2020-11-21
This natural variation can be used to identify genes controlling this trait by means of quantitative trait loci (QTL) mapping.
In this chapter, we describe how QTL mapping for seed dormancy inArabidopsis thaliana can be performed and how QTL analyses can be used to eventually identify the causal gene.
-
Construction of Infectious Clones for RNA Viruses: TMV
Time of Update: 2020-11-21
The generation of infectious clones is routinely the first step for reverse genetic studies of RNA plant virus gene and sequence function.
The procedure given here, details the creation of c DNA clones of tobacco mosaic virus, from which infectious transcripts can be generated in vitro with T7 RNA polymerase.
-
Isotopomer Measurement Techniques in Metabolic Flux Analysis II: Mass Spectrometry
Time of Update: 2020-11-21
Mass spectrometry (MS) offers a sensitive, reliable, and highly accurate method for measurement of isotopic labeling, which is required for generating comprehensive flux maps using metabolic flux analysis (MFA).
We describe the steps of sample preparation, MS analysis, and data handling required to obtain high-quality isotope labeling measurements that are applicable to MFA.
-
Detection of Plant RNA Viruses by Nonisotopic Dot-Blot Hybridization
Time of Update: 2020-11-21
Until now, methods based on the protein component of the viral particle have been mainly used in plant virus detection ( 1 , 2 ).
The application of recombinantDNA technology to plant virology has permitted the use of diagnostic methods based on the genomic component of viruses.
-
Extraction and Purification of an Enzyme Potentially Involved in ABA Biosynthesis
Time of Update: 2020-11-21
Such manipulation is very dependent on a detailed knowledge of the biosynthetic pathways involved in the production of the hormones, and more precisely about the enzymes involved in this biosynthesis.
-
Transformation of Maize by Electroporation of Embryos
Time of Update: 2020-11-21
Transformation methods that force DNA through this network of cellulose fibers include particle gun technology (ballistics; 1 ), imbibition of dried seeds ( 2 ), and use of silicon carbide “whiskers” ( 3 ).
-
Molecular Markers for Genetics and Plant Breeding: The MFLP Marker System and Its Application in Narrow-Leafed Lupin (Lupinus angustifolius)
Time of Update: 2020-11-21
Since the development of molecular markers to tag genes of agronomic traits of interests, molecular markers have played an increasingly significant role in breeding programs.
Molecular markers have been implemented for large-scale marker-assisted selection in the breeding program of many important crops including lupin.
-
An Improved Method for Plant BAC Library Construction
Time of Update: 2020-11-21
As the costs of positional cloning, physical mapping, and genome sequencing continuously decrease, there is an increasing demand for high-quality deep-coverage large insert BAC libraries.
In our laboratory, we have constructed many high-quality deep-coverage large insert BAC libraries including arabidopsis, manocot and dicot crop plants, and plant pathogens.
-
Forward Genetic Screening of Insertional Mutants
Time of Update: 2020-11-21
Protocols for the identification and confirmation of mutations by forward genetics, the isolation of insertion/genome junctions, and the stabilization of transposon-induced mutations are provided, together with an overview of available insertional mutant collections and of their characteristics.
-
Imaging Plant Nuclei and Membrane-Associated Cytoskeleton by Field Emission Scanning Electron Microscopy
Time of Update: 2020-11-21
This protocol shows how to isolate plant nuclei for SEM imaging of the nuclear envelope and associated structures from both sides of the nuclear envelope in cultured cells as well as in leaf or root cells.
-
NMR Analysis of Viral Protein Structures
Time of Update: 2020-11-21
Nuclear magnetic resonance ( NMR ) spectroscopy is a powerful tool to study the three-dimensional structure of proteins and nucleic acids at atomic resolution.
In addition to structure determination, NMR applications can investigate time-dependent phenomena, such as dynamic features of the biomacromolecules, reaction kinetics, molecular recognition, or protein folding.
-
Elucidation of Metabolic Pathways from Enzyme Classification Data
Time of Update: 2020-11-21
AReactions Database has been created from the material in theEnzyme List to allow reactions to be searched by substrate/product, and pathways to be traced from any selected starting/seed substrate.
-
Plant Viroids: Isolation, Characterization/Detection, and Analysis
Time of Update: 2020-11-21
Polyacrylamide gel electrophoresis (PAGE) was the first molecular technique to be used for the rapid (2–3 days) identification of viroid-infected plants.
Because it is the only diagnostic method that is sequence-independent, PAGE under denaturing conditions continues to play a key role in the identification of new viroids.
-
Callus and Suspension Culture Induction, Maintenance, and Characterization
Time of Update: 2020-11-21
Callus and cell suspension can be used for long-term cell cultures maintenance.
Methods for plant regeneration and growth kinetics of garlic cultures are described, as well as for cell viability of NT-1 cells stained with 2,3,5 triphenyltetrazolium chloride.
-
Molecular Analysis of Transgenic Rice
Time of Update: 2020-11-21
It makes little sense to invest time and effort in assaying biological activities of transgenic plants if the plants do not contain a functional gene of interest.
