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Callus and Suspension Culture Induction, Maintenance, and Characterization

 Last Update: 2020-11-21
 Source: Internet
 Author: User

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hep 2 cells

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Callus and cell suspension can be used for long-term cell cultures maintenance. This chapter describes procedures for the induction of somatic embryos of garlic, keeping a regeneration capacity for more than 5 yr, as well as the maintenance of a tobacco suspension culture (NT-1 cells), for more than 10 yr. Methods for plant regeneration and growth kinetics of garlic cultures are described, as well as for cell viability of NT-1 cells stained with 2,3,5 triphenyltetrazolium chloride. The packed cell volume determination as a parameter of growth is detailed.

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Callus, Suspension Culture, and Hairy Roots. Induction, Maintenance and Characterization

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Callus and Suspension Culture Induction, Maintenance, and Characterization

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Callus, Suspension Culture, and Hairy Roots. Induction, Maintenance and Characterization