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Incorporation and Analysis of LHCII in Model Systems
Time of Update: 2020-11-21
Understanding photophysical processes in this pigment-protein complex and the relationship between the structure and function of LHCII has been the subject of numerous studies, including those in the model systems, such as monomolecular layers, Langmuir-Blodgett films, planar lipid bilayers, and liposomes.
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In Situ Hybridization to RNA in Whole Arabidopsis Plants
Time of Update: 2020-11-21
(5 ) first developed an in situ hybridization protocol to detect cauliflower mosaic virus (CaMV) DNA m individual turnip leaves, and Leisner et al.
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Prevention and Control of Pests and Diseases
Time of Update: 2020-11-21
Therefore, regular scouting for infestations, frequent cleaning of plant growth areas, proper disposal of dead or diseased plant material, and controlled access to the greenhouses or growth chambers will help to make experiments more reproducible.
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Laser-Capture Microdissection to Study Global Transcriptional Changes During Plant Embryogenesis
Time of Update: 2020-11-21
Mutagenic approaches have proved powerful to identify essential genes, but global, transcriptome-wide analysis of mRNA profiles in cells at different stages of differentiation would allow the identification of changes in the abundance of major classes of transcripts expressed from genes that are known to respond to regulatory signals, such as hormones.
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Heterologous Expression in Yeast
Time of Update: 2020-11-21
The complex nature of plant metabolism often can seriously hinder the functional analysis of many proteins; and this has led some researchers to attempt to express such proteins in less sophisticated organisms.
The yeastSaccharomyces cerevisiae , however, has a combination of properties that make it a particularly attractive system for the functional expression of plant proteins.
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Introduction to Classical Crossprotection
Time of Update: 2020-11-21
In the late 1920s, it became apparent that, when plants were deliberately inoculated with two agents causing different types of visible symptom, there could be a form of interference.
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Methods for Isolation of Total RNA to Recover miRNAs and Other Small RNAs from Diverse Species
Time of Update: 2020-11-21
In this chapter, we will present a set of total RNA isolation methods that can be used to maximize the recovery of high-quality RNA to be used in miRNA analysis for a large number of plant species and tissue types.
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Genome Sequencing Approaches and Successes
Time of Update: 2020-11-21
The sequence of a genome is often referred to as the genetic blueprint, and is the foundation for all additional information from the genome to the phenome.
This will increase our understanding of how differences in the sequence relate to phenotypic observations, heritable traits, speciation, and evolution.
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No earthy potted plants
Time of Update: 2020-11-21
In this way, during plant growth, the root system will be from the bottom of the inner box hole into the outer basin, through the wet area, into the nutrient solution, this potted plant way, so that water, nutrients and oxygen can meet the needs of the root system.
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In Vitro Transcription of Class II Promoters in Higher Plants
Time of Update: 2020-11-21
Class II gene in vitro transcription systems from animal cells ( 1 – 3 ) or fungi ( 4 ) have been essential for elucidating the mechanisms regulating gene expression. Crude protein extracts from Hela
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Potato Cultivar Genome Analysis
Time of Update: 2020-11-21
Identification of cultivars by morphological characteristics is a highly skilled and time-consuming task, and for these reasons a rapid and robust method for variety differentiation has become extremely desirable.
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Probing ProteinProtein Interactions with FRETFLIM
Time of Update: 2020-11-21
The quantification of molecular interactions or conformational changes can conveniently be studied by using F�rster Resonance Energy Transfer (FRET) as a spectroscopic ruler. The FRET phenomenon desc
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Methylation-Sensitive Amplified Polymorphism (MSAP) Marker to Investigate Drought-Stress Response in Montepulciano and Sangiovese Grape Cultivars
Time of Update: 2020-11-21
Methylation-sensitive amplified polymorphism (MSAP) is a technique developed for assessing the extent and pattern of cytosine methylation and has been applied to genomes of several species (Arabidops
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Quantitative Trait Locus Analysis as a Gene Discovery Tool
Time of Update: 2020-11-21
Quantitative trait locus analysis has been a mainstay approach for obtaining a genetic description of complex agronomic traits for plants.
What is sometimes overlooked is the role quantitative trait locus (QTL) analysis can play in identifying genes that underlay complex traits.
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Davidia involucrata (Davidia involucrata)
Time of Update: 2020-11-21
is one of the first species of the Geminal Leaf Plant Gang. Up to 20, meters, deciduous trees, leaf paper, mutual, no leaves, dense on the top of the branches, broad egg shape or near the circle,
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Growth of Other Species Related to Arabidopsis thaliana
Time of Update: 2020-11-21
This chapter attempts to provide sources for acccessions and standard protocols for plant growth and seed harvest of wild crucifers while providing special tips for species with which we have experience.
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Development and Use of OatMaize Chromosome Additions and Radiation Hybrids
Time of Update: 2020-11-21
However, a minority of the offspring retain one or more maize chromosome(s) in addition to their haploid oat complements (partial hybrids).
Controlled self-fertilization of partial hybrids allows for the production of doubled haploid oat plants with an added single maize chromosome (monosomic addition) or an added pair of homologous maize chromosomes (disomic addition) among the inbred offspring.
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Betanin Production and Release In Vitro from Suspension Cultures of Beta vulgaris
Time of Update: 2020-11-21
Although annual crops are common for the production of such compounds, it is also often the case that the time required for field growth of a plant may be several years, 7-16 yr in the case of Cinchona, 5-7 yr in the case of the shikonin-yielding root of Lithospermum erythrorhizon (3).
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Cloning Genes from T-DNA Tagged Mutants
Time of Update: 2020-11-21
Insertion mutagenesis has a major advantage over other types of mutagenesis in that it not only causes a disruption of the gene, but the insertion element serves as a vehicle for recovery of the flankingDNA .
A few of the advantages of T-DNAs are that they are:
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Rapid Cloning of Genes and Promoters for Functional Analyses
Time of Update: 2020-11-21
Unlike traditional cloning approaches that involve subcloning or a special recipient vector and special flanking sequences, this protocol describes a rapid and cost-effective method for the direct insertion into the vector of choice.
